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Journal of Clinical Microbiology, October 2002, p. 3789-3792, Vol. 40, No. 10
0095-1137/02/$04.00+0     DOI: 10.1128/JCM.40.10.3789-3792.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.

Mutations in the rpoB and katG Genes Leading to Drug Resistance in Mycobacterium tuberculosis in Latvia

T. Tracevska,1* I. Jansone,1 L. Broka,2 O. Marga,3 and V. Baumanis1

Laboratory of Molecular Microbiology, Biomedical Research and Study Centre, University of Latvia,1 Latvian State Center for Tuberculosis and Lung Diseases, District of Riga,2 Latvian Academy of Medicine, Riga, Latvia3

Received 29 March 2002/ Returned for modification 21 June 2002/ Accepted 12 July 2002

To characterize the genetic basis of drug resistance in Mycobacterium tuberculosis in Latvia, mutations involved in rifampin (rpoB gene) and isoniazid (katG gene) resistance in DNA from 19 drug-susceptible and 51 multidrug-resistant M. tuberculosis complex isolates were analyzed. The most frequent rpoB gene mutations found by the Line Probe assay were the S531L (14 of 34 isolates), D516V (7 of 34), H526D (4 of 34), and D516Y plus P535S (4 of 34) mutations. Direct sequencing of seven isolates with unclear results from Line Probe assay showed the presence of the L533P mutation and the Q510H plus H526Y (1 of 34) and D516V plus P535S (4 of 34) double mutations, neither of which has been described previously. Single-strand conformation polymorphism analysis showed strand mobility differences between the rifampin-susceptible and -resistant samples for the D516V, H526D, and D516Y plus P535S mutations but not for the S531L mutation. Nucleotide substitution at codon 315 (AGC->ACC) of the katG gene was found in 48 of 51 multidrug-resistant samples by sequencing. Furthermore, katG gene restriction fragment length polymorphism analysis with endonuclease AciI confirmed the nucleotide change in codon 315.

* Corresponding author. Mailing address: Laboratory of Molecular Microbiology, Biomedical Research and Study Centre, University of Latvia, Ratsupites 1, Riga, LV-1067 Latvia. Phone: 371-7808218. Fax: 371-7442407. Email: tatjanap{at}biomed.lu.lv.

Journal of Clinical Microbiology, October 2002, p. 3789-3792, Vol. 40, No. 10
0095-1137/02/$04.00+0     DOI: 10.1128/JCM.40.10.3789-3792.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.



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