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Journal of Clinical Microbiology, October 2002, p. 3854-3856, Vol. 40, No. 10
0095-1137/02/$04.00+0     DOI: 10.1128/JCM.40.10.3854-3856.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.

Longitudinal Quantitative Detection by Real-Time PCR of Mycoplasma genitalium in First-Pass Urine of Men with Recurrent Nongonococcal Urethritis

Takashi Deguchi,1* Takashi Yoshida,2 Shigeaki Yokoi,1 Masayasu Ito,1 Masayoshi Tamaki,3 Hiroaki Ishiko,2 and Shin-Ichi Maeda3

Department of Urology, Gifu University School of Medicine, Gifu 500-8705,1 Research and Development Department, Mitsubishi Kagaku Bio-Clinical Laboratories, Inc., Tokyo 174-8555,2 Department of Urology, Toyota Memorial Hospital, Toyota 471-8513, Japan3

Received 1 April 2002/ Returned for modification 21 June 2002/ Accepted 14 July 2002

By using a TaqMan assay we monitored longitudinal changes in Mycoplasma genitalium loads in five men with recurrent M. genitalium-positive nongonococcal urethritis. We observed regrowth of M. genitalium persisting in hosts after treatment and a possible association of the increase in the M. genitalium load with emergence of symptoms and signs of nongonococcal urethritis in four of these patients.

* Corresponding author. Mailing address: Department of Urology, Gifu University School of Medicine, 40 Tsukasa-Machi, Gifu City, Gifu 500-8705, Japan. Phone: 81-58-265-1241. Fax: 81-58-265-9009. E-mail: deguchit{at}cc.gifu-u.ac.jp.

Journal of Clinical Microbiology, October 2002, p. 3854-3856, Vol. 40, No. 10
0095-1137/02/$04.00+0     DOI: 10.1128/JCM.40.10.3854-3856.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.



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