This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by De Baere, T.
Right arrow Articles by Vaneechoutte, M.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by De Baere, T.
Right arrow Articles by Vaneechoutte, M.

 Previous Article  |  Next Article 

Journal of Clinical Microbiology, October 2002, p. 3867-3870, Vol. 40, No. 10
0095-1137/02/$04.00+0     DOI: 10.1128/JCM.40.10.3867-3870.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.

CASE REPORT

Isolation of Buttiauxella gaviniae from a Spinal Cord Patient with Urinary Bladder Pathology

Thierry De Baere,1 Georges Wauters,2 Peter Kämpfer,3 Caroline Labit,1 Geert Claeys,1 Gerda Verschraegen,1 and Mario Vaneechoutte1*

Department of Microbiology, Ghent University Hospital, Ghent,1 Department of Microbiology, Université Catholique de Louvain, Brussels, Belgium,2 Institut für angewändte Mikrobiologie, Justus Liebig Universität, Giessen, Germany3

Received 3 May 2002/ Returned for modification 27 June 2002/ Accepted 23 July 2002

ABSTRACT

A gram-negative Buttiauxella gaviniae-like organism (LBV449) was isolated from a urine sample of a patient suffering from urine bladder pathology and neurological problems. The isolate was positive for adonitol fermentation and L-arginine dihydrolase and negative for melibiose and L-ornithine decarboxylase. The API 20E code was 3004113. Retrospectively, another isolate (ENT107), from a leg wound, was recovered from our collections and was shown to have similar biochemical characteristics. DNA-DNA hybridization showed 77% similarity between both strains, and strain LBV449 revealed 74% DNA-DNA similarity to the type strain of B. gaviniae. Neither 16S rRNA gene sequencing nor fatty acid analysis were useful for identification. The characteristic tRNA-PCR patterns obtained for these two clinical isolates consisted of fragments with lengths of 102.2, 105.4, 116.6, and 136.9 bp and most resembled the tRNA-PCR pattern obtained for B. gaviniae, but they lacked the B. gaviniae fragments of 88.2 and 239.5 bp. To our knowledge, no clinical cases with Buttiauxella strains have been described thus far.

* Corresponding author. Mailing address: Laboratory Bacteriology & Virology, Blok A, Ghent University Hospital, De Pintelaan 185, B9000 Ghent, Belgium. Phone: 32 9 240 36 92. Fax: 32 9 240 36 59. E-mail: Mario.Vaneechoutte{at}rug.ac.be.

Journal of Clinical Microbiology, October 2002, p. 3867-3870, Vol. 40, No. 10
0095-1137/02/$04.00+0     DOI: 10.1128/JCM.40.10.3867-3870.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.





Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»