This Article Full Text Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Reprints and Permissions Copyright Information Books from ASM Press MicrobeWorld Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Mochizuki, M. Articles by Carmichael, L. E. Search for Related Content PubMed PubMed Citation Articles by Mochizuki, M. Articles by Carmichael, L. E.

 Previous Article  |  Next Article 

Journal of Clinical Microbiology, November 2002, p. 3993-3998, Vol. 40, No. 11
0095-1137/02/$04.00+0     DOI: 10.1128/JCM.40.11.3993-3998.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.

Virologic and Serologic Identification of Minute Virus of Canines (Canine Parvovirus Type 1) from Dogs in Japan

Masami Mochizuki,^1* Michiru Hashimoto,¹ Takayuki Hajima,¹ Mitsuyoshi Takiguchi,² Akira Hashimoto,² Yumi Une,³ Frank Roerink,⁴ Takahisa Ohshima,⁴ Colin R. Parrish,⁵ and Leland E. Carmichael⁵

Laboratory of Clinical Microbiology,¹ Tsukuba Central Laboratories, Kyoritsu Seiyaku Corporation, Chiyoda-ku, Tokyo 102-0073,⁴ Laboratory of Pathobiology, Department of Veterinary Clinical Sciences, Graduate School of Veterinary Medicine, Hokkaido University, Sapporo 060-0818,² Laboratory of Veterinary Pathology, School of Veterinary Medicine, Azabu University, Sagamihara 229-8501, Japan,³ James A. Baker Institute, New York State College of Veterinary Medicine, Cornell University, Ithaca, New York 14853⁵

Received 4 March 2002/ Returned for modification 19 June 2002/ Accepted 22 July 2002

Minute virus of canines (MVC), also known as canine parvovirus type 1, was initially believed to be a nonpathogenic agent, since it was first isolated from canine fecal specimens in the late 1960s. However, subsequent pathological as well as epidemiological studies suggested that MVC is a pathogen of neonatal puppies and is widely distributed among domestic dogs in the United States. The virus also has been shown to cause fetal deaths. Nevertheless, the virus was not detected in dogs outside the United States until recently, presumably because of a lack of widespread availability of the only susceptible canine cell line, WRCC/3873D, used for MVC isolation. We examined 470 clinical specimens from 346 dogs by PCR and detected MVC-specific gene fragments from four diseased puppies (positive rate, 1.2%). Viruses were recovered from three PCR-positive rectal specimens by using WRCC/3873D and MDCK cells. The isolates possessed antigenic and genomic properties similar to those of the U.S. reference strain GA3 and were identified as MVC. In addition, seroepidemiological evidence that 5.0% of dogs possessed anti-MVC antibodies also indicated the presence of MVC infection among dogs in Japan. From this study and several recent European reports describing MVC field cases, it is evident that MVC is distributed among domestic dogs worldwide.

---

* Corresponding author. Mailing address: Laboratory of Clinical Microbiology, Kyoritsu Seiyaku Corporation, 1-12-4 Kudankita, Chiyoda-ku, Tokyo 102-0073, Japan. Phone: 81-3-3264-7117. Fax: 81-3-3264-6094. E-mail: msmmchzk{at}mb.infoweb.ne.jp.

---

Journal of Clinical Microbiology, November 2002, p. 3993-3998, Vol. 40, No. 11
0095-1137/02/$04.00+0     DOI: 10.1128/JCM.40.11.3993-3998.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.

This article has been cited by other articles:

• Sun, Y., Chen, A. Y., Cheng, F., Guan, W., Johnson, F. B., Qiu, J. (2009). Molecular Characterization of Infectious Clones of the Minute Virus of Canines Reveals Unique Features of Bocaviruses. J. Virol. 83: 3956-3967 [Abstract] [Full Text]