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Journal of Clinical Microbiology, November 2002, p. 4161-4165, Vol. 40, No. 11
0095-1137/02/$04.00+0     DOI: 10.1128/JCM.40.11.4161-4165.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.

Evaluation of an Enzyme-Linked Immunosorbent Assay (ELISA) with Affinity-Purified Em18 and an ELISA with Recombinant Em18 for Differential Diagnosis of Alveolar Echinococcosis: Results of a Blind Test

Akira Ito,1* Ning Xiao,1,2 Martine Liance,3 Marcello O. Sato,1 Yasuhito Sako,1 Wulamu Mamuti,1,4 Yuji Ishikawa,1 Minoru Nakao,1 Hiroshi Yamasaki,1 Kazuhiro Nakaya,5 Karine Bardonnet,6 Solange Bresson-Hadni,6 and Dominique A. Vuitton6

Department of Parasitology,1 Animal Laboratory for Medical Research, Asahikawa Medical College, Asahikawa, Japan,5 Sichuan Institute of Parasitic Diseases, Chengdu,2 Department of Parasitology, Xinjiang Medical University, Urumqi, China,4 Laboratoire de Parasitologie-Mycologie, Hôpital Henri Mondor AP-HP, et Université Paris 12, Paris,3 Université de Franche-Comté, WHO Collaborating Center on Prevention and Treatment of Human Echinococcosis, Besançon, France6

Received 3 May 2002/ Returned for modification 9 July 2002/ Accepted 30 July 2002

Alveolar echinococcosis (AE) is the most potentially lethal parasitic zoonosis of the nontropical areas in the northern hemisphere, where cystic echinococcosis (CE) is also endemic. Both AE and CE are highly endemic in China, and both serologic detection of echinococcosis, either AE or CE, and differentiation of AE from CE are crucial problems. Evaluation of Western blot analysis (WB) and enzyme-linked immunosorbent assay (ELISA) for the Em18 antigen, using affinity-purified and recombinant Em18, was carried out "blindly" using 60 human sera from patients diagnosed in France. The results were compared with those obtained using a commercially available Echinococcus WB immunoglobulin G (IgG) kit developed in France. The Em18 WB and Echinococcus WB IgG showed very similar results for detection of AE. Both affinity-purified Em18 or a recombinant Em18 WB and Echinococcus WB IgG seem useful for identification of AE, and the latter seems appropriate for both AE and CE, whereas affinity-purified Em18 ELISA and the newly developed recombinant Em18 ELISA appear to be suitable for detection of AE, especially for epidemiological surveys.

* Corresponding author. Mailing address: Department of Parasitology, Asahikawa Medical College, Midorigaoka-Higashi 2-1-1-1, Asahikawa 078-8510, Japan. Phone: 81-166-68-2420. Fax: 81-166-68-2429. E-mail: akiraito{at}asahikawa-med.ac.jp.

Journal of Clinical Microbiology, November 2002, p. 4161-4165, Vol. 40, No. 11
0095-1137/02/$04.00+0     DOI: 10.1128/JCM.40.11.4161-4165.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.



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