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Journal of Clinical Microbiology, November 2002, p. 4281-4284, Vol. 40, No. 11
0095-1137/02/$04.00+0     DOI: 10.1128/JCM.40.11.4281-4284.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.

Presumptive Identification of Candida kefyr on Levine Formulation of Eosin Methylene Blue Agar

Erik L. Munson,1,{dagger} Dennis R. Troy,1 Joanne K. Weber,2 Shawn A. Messer,1 and Michael A. Pfaller1*

Medical Microbiology Division, Department of Pathology, University of Iowa College of Medicine, Iowa City, Iowa 52242,1 Department of Medical Microbiology and Immunology, University of Wisconsin Medical School, Madison, Wisconsin 537062

Received 4 April 2002/ Returned for modification 28 May 2002/ Accepted 30 July 2002

Three hundred thirty-one yeast and yeast-like isolates were cultivated on eosin methylene blue agar. While the sensitivity rate for Candida kefyr isolates producing a metallic green sheen was 81.8%, the high positive predictive value (100%) for yeast isolates with this phenotype belonging to C. kefyr suggests that these isolates can be presumptively identified as C. kefyr.


* Corresponding author. Mailing address: University of Iowa College of Medicine, Department of Pathology, Clinical Microbiology Laboratories, 200 Hawkins Dr., C606B GH, Iowa City, IA 52242. Phone: (319) 384-9566. Fax: (319) 356-4916. E-mail: michael-pfaller{at}uiowa.edu.

{dagger} Present address: Medical Science Laboratories, Wauwatosa, WI 53226.


Journal of Clinical Microbiology, November 2002, p. 4281-4284, Vol. 40, No. 11
0095-1137/02/$04.00+0     DOI: 10.1128/JCM.40.11.4281-4284.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.