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Journal of Clinical Microbiology, November 2002, p. 4346-4348, Vol. 40, No. 11
0095-1137/02/$04.00+0     DOI: 10.1128/JCM.40.11.4346-4348.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.

Quantification of Hepatitis C Virus in Human Liver and Serum Samples by Using LightCycler Reverse Transcriptase PCR

Peter A. White,1,2,3* Yong Pan,1 Anthony J. Freeman,4 George Marinos,4 Rosemary A. Ffrench,5 Andrew R. Lloyd,3 and William D. Rawlinson1,2,3

Virology Division, Department of Microbiology, SEALS,1 Viral Hepatitis Research, Gastrointestinal and Liver Unit, Prince of Wales Hospital,4 Department of Immunology and Infectious Diseases, Sydney Children's Hospital, Sydney, New South Wales 2031,5 School of Biotechnology and Biomolecular Sciences, Faculty of Science,2 School of Medical Science, Faculty of Medicine, The University of New South Wales, Sydney, New South Wales 2052, Australia3

Received 12 June 2002/ Accepted 8 August 2002

A highly sensitive, non-probe-based, real-time quantitative reverse transcriptase PCR was developed for viral load measurements in both serum and liver samples from patients with hepatitis C virus (HCV) infection. With synthetic RNA, the linearity of the approach was conserved over a wide range of HCV copy numbers. There was a strong correlation between hepatic and serum viral load measurements (r = 0.689, P = 0.004, n = 15), indicating that the level of viremia reflected the amount of virus present in the liver.

* Corresponding author. Mailing address: Virology Division, Department of Microbiology, SEALS, Prince of Wales Hospital, Randwick, NSW 2031, Australia. Phone: 61 2 9382 9096. Fax: 61 2 9398 4275. E-mail: whitepa{at}sesahs.nsw.gov.au.

Journal of Clinical Microbiology, November 2002, p. 4346-4348, Vol. 40, No. 11
0095-1137/02/$04.00+0     DOI: 10.1128/JCM.40.11.4346-4348.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.



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