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Journal of Clinical Microbiology, November 2002, p. 4360-4362, Vol. 40, No. 11
0095-1137/02/$04.00+0     DOI: 10.1128/JCM.40.11.4360-4362.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.

PCR-Based Detection of Bacillus anthracis in Formalin-Fixed Tissue from a Patient Receiving Ciprofloxacin

Steven M. Levine,1 Guillermo Perez-Perez,1,2 Asalia Olivares,1 Herman Yee,3 Bruce A. Hanna,2,3 and Martin J. Blaser1,2,4*

Departments of Medicine,1 Microbiology,2 Pathology, New York University School of Medicine,3 Veterans Affairs Medical Center, New York, New York4

Received 26 April 2002/ Returned for modification 11 July 2002/ Accepted 5 August 2002

We demonstrate that Bacillus anthracis may be detected from a formalin-fixed, paraffin-embedded biopsy specimen, even after the patient has received antibiotic treatment. Although traditional PCR methods may not be sufficiently sensitive for anthrax detection in such patients, cycle numbers can be increased or PCR can be repeated by using an aliquot from a previous PCR as the template.

* Corresponding author. Mailing Address: Department of Medicine, 550 First Ave., New York, NY 10016. Phone: (212) 263-6394. Fax: (212) 263-7700. E-mail: martin.blaser{at}med.nyu.edu.

Journal of Clinical Microbiology, November 2002, p. 4360-4362, Vol. 40, No. 11
0095-1137/02/$04.00+0     DOI: 10.1128/JCM.40.11.4360-4362.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Chensue, S. W. (2003). Exposing a Killer: Pathologists Angle for Anthrax. Am. J. Pathol. 163: 1699-1702 [Full Text]  


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