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Journal of Clinical Microbiology, December 2002, p. 4744-4747, Vol. 40, No. 12
0095-1137/02/$04.00+0     DOI: 10.1128/JCM.40.12.4744-4747.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.

Colony Multiplex PCR Assay for Identification and Differentiation of Campylobacter jejuni, C. coli, C. lari, C. upsaliensis, and C. fetus subsp. fetus

Gehua Wang,* Clifford G. Clark, Tracy M. Taylor, Chad Pucknell, Connie Barton, Lawrence Price, David L. Woodward, and Frank G. Rodgers{dagger}

National Laboratory for Enteric Pathogens, National Microbiology Laboratory, Winnipeg, Canada

Received 26 April 2002/ Returned for modification 30 July 2002/ Accepted 13 September 2002

A multiplex PCR assay was used to simultaneously detect genes from the five major clinically relevant Campylobacter spp. Those genes selected were hipO and 23S rRNA from Campylobacter jejuni; glyA from each of C. coli, C. lari, and C. upsaliensis; and sapB2 from C. fetus subsp. fetus. The assay was evaluated with 137 clinical and environmental isolates and was found to be rapid and easy to perform and had a high sensitivity and specificity for characterizing isolates, even in mixed cultures.

* Corresponding author. Mailing address: Special Project Unit, National Laboratory for Enteric Pathogens, National Microbiology Laboratory, 1015 Arlington St., Winnipeg, Manitoba, R3E 3R2, Canada. Phone: (204)789-6077. Fax: (204) 789-2018. E-mail: gehua_Wang{at}hc-sc.gc.ca.

{dagger} Present address: Department of Microbiology, Rudman Hall, University of New Hampshire, Durham, NH 03824.

Journal of Clinical Microbiology, December 2002, p. 4744-4747, Vol. 40, No. 12
0095-1137/02/$04.00+0     DOI: 10.1128/JCM.40.12.4744-4747.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.



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