This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Díez, S.
Right arrow Articles by Hamilton, A. J.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Díez, S.
Right arrow Articles by Hamilton, A. J.

 Previous Article  |  Next Article 

Journal of Clinical Microbiology, February 2002, p. 359-365, Vol. 40, No. 2
0095-1137/01/$04.00+0     DOI: 10.1128/JCM.40.2.359-365.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.

Paracoccidioides brasiliensis 87-Kilodalton Antigen, a Heat Shock Protein Useful in Diagnosis: Characterization, Purification, and Detection in Biopsy Material via Immunohistochemistry

Soraya Díez,1,2* Beatriz L. Gómez,1,2 Angela Restrepo,2 Rod J. Hay,1 and Andrew J. Hamilton1

St. John's Institute of Dermatology, Guy's Hospital, London, England,1 Corporación para Investigaciones Biológicas, Medellín, Colombia2

Received 9 July 2001/ Returned for modification 23 August 2001/ Accepted 21 October 2001

The 87-kDa antigen derived from the fungal pathogen Paracoccidioides brasiliensis can be detected in the sera of infected patients, and its levels have been shown to correlate well with response to treatment and with clinical cure. Despite its potential importance, the antigen has been poorly characterized. The 87-kDa antigen was purified to homogeneity via preparative gel electrophoresis; N-terminal amino acid sequencing revealed substantial homology with heat shock proteins (hsps) from a variety of organisms. A monoclonal antibody (MAb) raised against a Histoplasma capsulatum 80-kDa hsp showed cross-reactivity to the purified 87-kDa antigen via Western blotting, and the 87-kDa-specific MAb P1B demonstrated that the antigen was expressed at higher levels in yeast than in mycelia by the same technique. Enzyme-linked immunosorbent assay and immunofluorescence reactivity using P1B confirmed increased expression of the 87-kDa antigen during the temperature-induced transformation of mycelia to yeast. Yeast-to-mycelium transformation was accompanied by a fall in expression, although the 87-kDa antigen was clearly constitutively expressed in both phases. Immunochemical staining of tissues from patients with MAb P1B who were infected with P. brasiliensis confirmed in vivo expression of the 87-kDa antigen by yeasts, and identification of this antigen via this method appears to be a useful adjunct to other methods used to diagnose paracoccidioidomycosis.

* Corresponding author. Mailing address: Dermatology Laboratory, St. John's Institute of Dermatology, 5th Floor, Thomas Guy House, Guy's Hospital, London SE1 9RT, United Kingdom. Phone: 0207 955 4663. Fax: 0207 955 2103. E-mail: soraya.diez{at}kcl.ac.uk.

Journal of Clinical Microbiology, February 2002, p. 359-365, Vol. 40, No. 2
0095-1137/01/$04.00+0     DOI: 10.1128/JCM.40.2.359-365.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Gonzalez, A., Gomez, B. L., Diez, S., Hernandez, O., Restrepo, A., Hamilton, A. J., Cano, L. E. (2005). Purification and Partial Characterization of a Paracoccidioides brasiliensis Protein with Capacity To Bind to Extracellular Matrix Proteins. Infect. Immun. 73: 2486-2495 [Abstract] [Full Text]  
  • Marques da Silva, S. H., de Mattos Grosso, D., Lopes, J. D., Colombo, A. L., Blotta, M. H. S. L., Queiroz-Telles, F., Pires de Camargo, Z. (2004). Detection of Paracoccidioides brasiliensis gp70 Circulating Antigen and Follow-Up of Patients Undergoing Antimycotic Therapy. J. Clin. Microbiol. 42: 4480-4486 [Abstract] [Full Text]  
  • Diez, S., Gomez, B. L., McEwen, J. G., Restrepo, A., Hay, R. J., Hamilton, A. J. (2003). Combined Use of Paracoccidioides brasiliensis Recombinant 27-Kilodalton and Purified 87-Kilodalton Antigens in an Enzyme-Linked Immunosorbent Assay for Serodiagnosis of Paracoccidioidomycosis. J. Clin. Microbiol. 41: 1536-1542 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»