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Journal of Clinical Microbiology, February 2002, p. 366-371, Vol. 40, No. 2
0095-1137/01/$04.00+0     DOI: 10.1128/JCM.40.2.366-371.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.

Engineered BGMK Cells for Sensitive and Rapid Detection of Enteroviruses

Yung T. Huang,1* Paul Yam,2 Huimin Yan,1 and Yan Sun1

Department of Pathology, University Hospitals of Cleveland, Case Western Reserve University, Cleveland, Ohio 44106,1 Children's Hospital of Omaha, Omaha, Nebraska 681142

Received 17 September 2001/ Returned for modification 12 October 2001/ Accepted 13 November 2001

Decay-accelerating factor (DAF) has been reported to be a cellular receptor for several enteroviruses. Buffalo green monkey kidney (BGMK) cells expressing human DAF (BGMK-hDAF cells) showed increased susceptibility and sensitivity to several types of enteroviruses compared to wild-type BGMK cells. When 17 frozen positive clinical samples were tested, BGMK cells detected 8 and BGMK-hDAF cells detected 16. Since the CaCo-2 cell line has been documented to support the replication of most enteroviruses, CaCo-2 cells were mixed with BGMK-hDAF cells in order to increase the number of viruses detected. Thirty-four frozen clinical samples that previously had tested positive for enteroviruses were tested, and the following numbers were detected: 33 of 34 by CaCo-2/BGMK-hDAF cells, 29 of 34 by CaCo-2/BGMK cells, 28 of 34 by H292/RD (E-mix A) and A-549/BGMK (E-mix B) cells, and 26 of 34 by MRC-5 and pRhMK cells.

* Corresponding author. Mailing address: Department of Pathology, University Hospitals of Cleveland, Case Western Reserve University, 2085 Adelbert Rd., Cleveland, OH 44106. Phone: (216) 844-8611. Fax: (216) 844-5601. E-mail: yth{at}po.cwru.edu.

Journal of Clinical Microbiology, February 2002, p. 366-371, Vol. 40, No. 2
0095-1137/01/$04.00+0     DOI: 10.1128/JCM.40.2.366-371.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.



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