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Journal of Clinical Microbiology, February 2002, p. 453-460, Vol. 40, No. 2
0095-1137/02/$04.00+0     DOI: 10.1128/JCM.40.02.453-460.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.

Species-Specific Serodiagnosis of Lyme Arthritis and Neuroborreliosis Due to Borrelia burgdorferi Sensu Stricto, B. afzelii, and B. garinii by Using Decorin Binding Protein A

Tero Heikkilä,1,2* Ilkka Seppälä,2,3 Harri Saxen,1 Jaana Panelius,2 Heta Yrjänäinen,4 and Pekka Lahdenne1

Hospital for Children and Adolescents,1 Department of Bacteriology and Immunology, Haartman Institute, University of Helsinki,2 HUCH-Laboratory Diagnostics, Helsinki University Central Hospital, Helsinki,3 Department of Medical Microbiology, University of Turku, Turku, Finland4

Received 19 March 2001/ Returned for modification 29 July 2001/ Accepted 14 October 2001

The antigenic potential of decorin binding protein A (DbpA) was evaluated in serodiagnosis of human Lyme borreliosis (LB). The dbpA was cloned and sequenced from the three pathogenic Borrelia species common in Europe. Sequence analysis revealed high interspecies heterogeneity. The identity of the predicted amino acid sequences was 43 to 62% among Borrelia burgdorferi sensu stricto, B. afzelii, and B. garinii. The respective recombinant DbpAs (rDbpAs) were produced and tested as antigens by Western blotting and enzyme-linked immunosorbent assay (ELISA). One hundred percent of patients with neuroborreliosis (NB) and 93% of patients with Lyme arthritis (LA) reacted positively. Sera from the majority of patients reacted with one rDbpA only and had no or low cross-reactivity to other two variant proteins. In patients with culture-positive erythema migrans (EM), the sensitivity of rDbpA immunoglobulin G (IgG) or IgM ELISA was low. The DbpA seems to be a sensitive and specific antigen for the serodiagnosis of LA or NB, but not of EM, provided that variants from all three pathogenic borrelial species are included in the combined set of antigens.

* Corresponding author. Mailing address: Department of Bacteriology and Immunology, Haartman Institute, Haartmaninkatu 3, FIN-00014 University of Helsinki, Finland. Phone: 358-9-1912 6395. Fax: 358-9-1912 6382. E-mail: tero.heikkila{at}helsinki.fi.

Journal of Clinical Microbiology, February 2002, p. 453-460, Vol. 40, No. 2
0095-1137/02/$04.00+0     DOI: 10.1128/JCM.40.02.453-460.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.



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