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Journal of Clinical Microbiology, February 2002, p. 547-552, Vol. 40, No. 2
0095-1137/02/$04.00+0     DOI: 10.1128/JCM.40.2.547-552.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.

Novel Antigens of Helicobacter pylori Correspond to Ulcer-Related Antibody Pattern of Sera from Infected Patients

Christo Atanassov,1* Leon Pezennec,1 Jacques d'Alayer,2 Ghislaine Grollier,1 Bertrand Picard,3 and Jean-Louis Fauchère1

Department of Microbiology A, IFR 59, University Hospital Center, 86021 Poitiers,1 Laboratory of Protein Microsequencing, Institut Pasteur, 75724 Paris,2 Laboratory of Microbiology, Faculty of Medicine, 29200 Brest, France3

Received 15 June 2001/ Returned for modification 24 September 2001/ Accepted 18 November 2001

Recently, we reported that the patterns of antibodies to Helicobacter pylori protein antigens in serum may be useful for screening patients at high risk for ulcers (P. Aucher et al., J. Clin. Microbiol. 36:931-936, 1998). Here we report the identification, by a combination of electrophoretic, immunochemical, and protein sequencing methods, of five antigens that correspond to this antibody pattern: groEL, catalase A, flagellin A, beta-ketoacyl-acyl carrier protein synthase I (ß-ketoacyl-ACP S), and peptidyl prolyl cis-trans isomerase (PPiase). ß-Ketoacyl-ACP S and PPiase are reported for the first time as antigens of diagnostic interest in infections by H. pylori. The antigenicity of the five antigens, together with those of CagA and VacA, was tested in an immunoblot assay with water-soluble protein extracts from two H. pylori pathogenic strains (HP 141 and ATCC 43579) and panels of sera from H. pylori-positive patients with gastroduodenal ulcers (GDU), nonulcer dyspepsia (NUD), as well as sera from H. pylori-negative healthy volunteers. For catalase A, groEL, and flagellin A antigens, no overall statistically important values were found making it possible to discriminate between patients with GDU and NUD. For both H. pylori strains, the mean performance indices (MPI) presenting percentages of correctly classified patients with GDU and NUD showed that the most significant antibody patterns were as follows: anti-VacA + anti-ß-ketoacyl-ACP S (MPI = 76.1), anti-VacA + anti-PPiase (MPI = 71.8), and anti-CagA + anti-VacA + anti-ß-ketoacyl-ACP S (MPI = 70.5). Antibody patterns detected with these antigen profiles may therefore be useful in developing a diagnostic test designed to predict the clinical severity of the H. pylori infection within the adult population of France.

* Corresponding author. Mailing address: Laboratoire de Microbiologie A, Centre Hospitalier Universitaire-La Miletrie, BP 577, 86021 Poitiers, France. Phone: 33-5-49-44-43-53. Fax: 33-5-49-44-38-88. E-mail: atanassov__christo{at}yahoo.fr.

Journal of Clinical Microbiology, February 2002, p. 547-552, Vol. 40, No. 2
0095-1137/02/$04.00+0     DOI: 10.1128/JCM.40.2.547-552.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.



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