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Journal of Clinical Microbiology, February 2002, p. 633-636, Vol. 40, No. 2
0095-1137/01/$04.00+0     DOI: 10.1128/JCM.40.02.633-636.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.

Selective Amplification of tyv (rfbE), prt (rfbS), viaB, and fliC Genes by Multiplex PCR for Identification of Salmonella enterica Serovars Typhi and Paratyphi A

Kenji Hirose,1* Ken-Ichiro Itoh,2 Hiroshi Nakajima,3 Takayuki Kurazono,4 Masanori Yamaguchi,4 Kazuo Moriya,5 Takayuki Ezaki,6 Yoshiaki Kawamura,6 Kazumichi Tamura,1 and Haruo Watanabe1

Department of Bacteriology, National Institute of Infectious Diseases, Tokyo 162-8640,1 Department of Microbiology, National Institute of Public Health, Tokyo 108-8638,2 Department of Microbiology, Okayama Prefectural Institute for Environmental Science and Public Health, Okayama 701-0298,3 Saitama Institute for Public Health, Saitama 338-0842,4 Saga Prefectural Institute of Public Health, Saga 849-0925,5 Department of Microbiology, Gifu University, School of Medicine, Gifu 500-8750, Japan6

Received 28 June 2001/ Returned for modification 26 July 2001/ Accepted 26 November 2001

The PCR primers for O, H, and Vi antigen genes, tyv (rfbE), prt (rfbS), fliC-d, fliC-a, and viaB, were designed and used for the rapid identification of Salmonella enterica serovars Typhi and Paratyphi A with multiplex PCR. The results showed that all the clinical isolates examined of Salmonella serovars Typhi and Paratyphi A were accurately identified by this assay.

* Corresponding author. Mailing address: Department of Bacteriology, National Institute of Infectious Diseases, 1-23-1 Toyama, Shinjuku, Tokyo 162-8640, Japan. Phone: 81-3-5285-1111, ext. 2230. Fax: 81-3-5285-1163. E-mail: hirosek{at}nih.go.jp.

Journal of Clinical Microbiology, February 2002, p. 633-636, Vol. 40, No. 2
0095-1137/01/$04.00+0     DOI: 10.1128/JCM.40.02.633-636.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.



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