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Journal of Clinical Microbiology, February 2002, p. 660-662, Vol. 40, No. 2
0095-1137/01/$04.00+0     DOI: 10.1128/JCM.40.2.660-662.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.

Use of Pulsed-Field Gel Electrophoresis, Enterobacterial Repetitive Intergenic Consensus Typing, and Automated Ribotyping To Assess Genomic Variability among Strains of Nontypeable Haemophilus influenzae

M. M. Pettigrew,1 B. Foxman,1 Z. Ecevit,2 C. F. Marrs,1 and J. Gilsdorf2*

Department of Epidemiology, University of Michigan, Ann Arbor, Michigan 48109-2029,,1 Department of Pediatrics and Communicable Diseases, University of Michigan, Ann Arbor, Michigan 48109-02442

Received 6 August 2001/ Returned for modification 27 September 2001/ Accepted 1 November 2001

We compared 75 nontypeable (NT) Haemophilus influenzae isolates by pulsed-field gel electrophoresis (PFGE), enterobacterial repetitive intergenic consensus (ERIC)-PCR, and automated ribotyping. PFGE was the most discriminatory of the techniques. ERIC-PCR provides a useful screen but should not replace other techniques as the sole method to group NT H. influenzae strains.

* Corresponding author. Mailing address: Department of Pediatrics and Communicable Diseases, University of Michigan, Ann Arbor, MI 48109-0244. Phone: (743) 763-2440. Fax: (743) 936-7635. E-mail: gilsdorf{at}umich.edu.

Journal of Clinical Microbiology, February 2002, p. 660-662, Vol. 40, No. 2
0095-1137/01/$04.00+0     DOI: 10.1128/JCM.40.2.660-662.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.



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