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Journal of Clinical Microbiology, February 2002, p. 663-665, Vol. 40, No. 2
0095-1137/01/$04.00+0 DOI: 10.1128/JCM.40.2.663-665.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.
Genotyping of Clinical Methicillin-Susceptible Staphylococcus aureus Isolates in a Dutch Teaching Hospital
Yvonne Van Dijk,1 Camiel L. C. Wielders,2 Ad C. Fluit,2 Armand Paauw,2 Rob J. A. Diepersloot,1 and Ellen M. Mascini1,2*
Departments of Infection Control and Microbiology and Immunology, Diakonessenhuis,1
Eijkman-Winkler Institute for Microbiology, Infectious Diseases, and Inflammation, Department of Hospital Hygiene and Infection Prevention, University Medical Center, Utrecht, The Netherlands2
Received 27 September 2001/
Returned for modification 18 November 2001/
Accepted 27 November 2001
Methicillin-susceptible Staphylococcus aureus isolates, recovered from 204 patients in our hospital in a 22-month period, were characterized by pulsed-field gel electrophoresis. Among the multiple S. aureus types six clonal lineages dominated, comprising isolates from 158 patients. Despite the limited genetic variation, cross-transmission was made plausible only sporadically.
* Corresponding author. Mailing address: Eijkman-Winkler Institute for Microbiology, Infectious Diseases, and Inflammation, University Medical Center G04.614, P.O. Box 85500, NL-3508 GA Utrecht, The Netherlands. Phone: 31-302-508784. Fax: 31-302-541770. E-mail:
e.m.mascini{at}lab.azu.nl.
Journal of Clinical Microbiology, February 2002, p. 663-665, Vol. 40, No. 2
0095-1137/01/$04.00+0 DOI: 10.1128/JCM.40.2.663-665.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.
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