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Journal of Clinical Microbiology, February 2002, p. 675-678, Vol. 40, No. 2
0095-1137/01/$04.00+0     DOI: 10.1128/JCM.40.2.675-678.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.

Quantification of Human Immunodeficiency Virus Type 1 Proviral DNA by Using TaqMan Technology

Yuqi Zhao,1,2,3,4* Min Yu,1 Johann W. Miller,2 Mingzhong Chen,1 Eric G. Bremer,1,5 William Kabat,1 and Ram Yogev1,3

Children's Memorial Institute for Education and Research,1 Center for Biotechnology, Northwestern University,2 Departments of Pediatrics,,3 Microbiology-Immunology ,4 Neurosurgery Northwestern University Medical School, Chicago, Illinois5

Received 14 May 2001/ Returned for modification 20 August 2001/ Accepted 24 November 2001

A protocol for quantification of human immunodeficiency virus type 1 (HIV-1) proviral DNA with the TaqMan technology was developed and validated. The assay was specific for HIV-1, with an analytic sensitivity of 10 copies and a linear dynamic range of >6 logs. Viral RNA levels, when at a stable state, were highly correlated with proviral DNA levels in 80 specimens of 18 HIV-infected children.

* Corresponding author. Mailing address: 2430 N. Halsted St., MB218, Chicago, IL 60614. Phone: (773) 880-6608. Fax: (773) 880-6609. E-mail: yzhao{at}northwestern.edu.

Journal of Clinical Microbiology, February 2002, p. 675-678, Vol. 40, No. 2
0095-1137/01/$04.00+0     DOI: 10.1128/JCM.40.2.675-678.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.



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