This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Ryan, J. R.
Right arrow Articles by Martin, S. K.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Ryan, J. R.
Right arrow Articles by Martin, S. K.

 Previous Article  |  Next Article 

Journal of Clinical Microbiology, March 2002, p. 1037-1043, Vol. 40, No. 3
0095-1137/02/$04.00+0     DOI: 10.1128/JCM.40.3.1037-1043.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.

Enzyme-Linked Immunosorbent Assay Based on Soluble Promastigote Antigen Detects Immunoglobulin M (IgM) and IgG Antibodies in Sera from Cases of Visceral and Cutaneous Leishmaniasis

Jeffrey R. Ryan,1* Anthony M. Smithyman,2 G-Halli Rajasekariah,2 Lisa Hochberg,1 John M. Stiteler,1 and Samuel K. Martin1

Entomology Dept., Division of Communicable Diseases and Immunology, Walter Reed Army Institute of Research, Silver Spring, Maryland,1 Cellabs Pty. Ltd., Sydney, Australia2

Received 31 July 2001/ Returned for modification 18 September 2001/ Accepted 11 December 2001

Leishmaniasis causes significant morbidity and mortality in areas where it is endemic. In areas where it is nonendemic, global travel and increased incidence of the disease in human immunodeficiency virus and intravenous-drug user populations are also causes for concern. The unavailability of rapid and reliable tests for diagnosis of the various leishmaniases makes patient management difficult. We have developed an enzyme-linked immunosorbent assay (ELISA) that can detect immunoglobulin M (IgM) and IgG antibodies in patients with visceral and cutaneous leishmaniasis. These practical assays are based on soluble antigens from promastigotes cultivated in a protein-free medium. In preliminary studies, 129 visceral (Brazil, Italy, North Africa, and Nepal) and 143 cutaneous (Brazil) leishmaniasis patients with controls were tested. Overall, the tests showed a sensitivity of 95.1%. In addition, the ELISA correctly identified 42 sera from Brazilian dogs with canine leishmaniasis and 10 healthy controls. Serological tests for the various clinical manifestations of leishmaniasis could be useful epidemiological and patient management tools in populations of areas of endemicity and nonendemicity.

* Corresponding author. Mailing address: Department of Entomology, Division of Communicable Diseases and Immunology, Walter Reed Army Institute of Research, Silver Spring, MD 20910-7500. Phone: (301) 319-9532. Fax: (301) 319-9012. E-mail: Jeffrey.Ryan{at}na.amedd.army.mil.

Journal of Clinical Microbiology, March 2002, p. 1037-1043, Vol. 40, No. 3
0095-1137/02/$04.00+0     DOI: 10.1128/JCM.40.3.1037-1043.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Marin, C., Longoni, S. S., Urbano, J., Minaya, G., Mateo, H., de Diego, J. A., Rosales, M. J., Perez-Cordon, G., Romero, D., Sanchez-Moreno, M. (2009). Enzyme-linked Immunosorbent Assay for Superoxide Dismutase-Excreted Antigen in Diagnosis of Sylvatic and Andean Cutaneous Leishmaniasis of Peru. Am J Trop Med Hyg 80: 55-60 [Abstract] [Full Text]  
  • Rajasekariah, G-H. R., Cardoso, L., Dogcio, D. A., Martin, S. K., Smithyman, A. M. (2008). A Novel Exo-antigen-based ELISA for the Detection of Canine Leishmaniasis. Am J Trop Med Hyg 78: 616-623 [Abstract] [Full Text]  
  • Zeyrek, F. Y., Korkmaz, M., Ozbel, Y. (2007). Serodiagnosis of Anthroponotic Cutaneous Leishmaniasis (ACL) Caused by Leishmania tropica in Sanliurfa Province, Turkey, Where ACL Is Highly Endemic. CVI 14: 1409-1415 [Abstract] [Full Text]  
  • TONUI, W. K., TITUS, R. G. (2007). CROSS-PROTECTION AGAINST LEISHMANIA DONOVANI BUT NOT L. BRAZILIENSIS CAUSED BY VACCINATION WITH L. MAJOR SOLUBLE PROMASTIGOTE EXOGENOUS ANTIGENS IN BALB/C MICE. Am J Trop Med Hyg 76: 579-584 [Abstract] [Full Text]  
  • Ozbilge, H., Aksoy, N., Gurel, M. S., Yazar, S. (2006). IgG and IgG subclass antibodies in patients with active cutaneous leishmaniasis.. J Med Microbiol 55: 1329-1331 [Abstract] [Full Text]  
  • RYAN, J. R., MBUI, J., RASHID, J. R., WASUNNA, M. K., KIRIGI, G., MAGIRI, C., KINOTI, D., NGUMBI, P. M., MARTIN, S. K., ODERA, S. O., HOCHBERG, L. P., BAUTISTA, C. T., CHAN, A. S. T. (2006). SPATIAL CLUSTERING AND EPIDEMIOLOGICAL ASPECTS OF VISCERAL LEISHMANIASIS IN TWO ENDEMIC VILLAGES, BARINGO DISTRICT, KENYA. Am J Trop Med Hyg 74: 308-317 [Abstract] [Full Text]  
  • Saha, S., Mazumdar, T., Anam, K., Ravindran, R., Bairagi, B., Saha, B., Goswami, R., Pramanik, N., Guha, S. K., Kar, S., Banerjee, D., Ali, N. (2005). Leishmania Promastigote Membrane Antigen-Based Enzyme-Linked Immunosorbent Assay and Immunoblotting for Differential Diagnosis of Indian Post-Kala-Azar Dermal Leishmaniasis. J. Clin. Microbiol. 43: 1269-1277 [Abstract] [Full Text]  
  • Tonui, W. K., Mejia, J. S., Hochberg, L., Mbow, M. L., Ryan, J. R., Chan, A. S. T., Martin, S. K., Titus, R. G. (2004). Immunization with Leishmania major Exogenous Antigens Protects Susceptible BALB/c Mice against Challenge Infection with L. major. Infect. Immun. 72: 5654-5661 [Abstract] [Full Text]  
  • Ravindran, R., Anam, K., Bairagi, B. C., Saha, B., Pramanik, N., Guha, S. K., Goswami, R. P., Banerjee, D., Ali, N. (2004). Characterization of Immunoglobulin G and Its Subclass Response to Indian Kala-Azar Infection before and after Chemotherapy. Infect. Immun. 72: 863-870 [Abstract] [Full Text]  
  • Sundar, S., Rai, M. (2002). Laboratory Diagnosis of Visceral Leishmaniasis. CVI 9: 951-958 [Full Text]  
  • Arya*, S. C (2002). Enzyme-Linked Immunosorbent Assays for Diagnosis of Leishmaniasis in Patients Coinfected with Human Immunodeficiency Virus. J. Clin. Microbiol. 40: 3110-3110 [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»