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Journal of Clinical Microbiology, March 2002, p. 1088-1090, Vol. 40, No. 3
0095-1137/02/$04.00+0     DOI: 10.1128/JCM.40.3.1088-1090.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.

Results of Human Papillomavirus DNA Testing with the Hybrid Capture 2 Assay Are Reproducible

Philip E. Castle,1* Attila T. Lorincz,2 Iwona Mielzynska-Lohnas,2 David R. Scott,3 Andrew G. Glass,4 Mark E. Sherman,1 John E. Schussler,5 and Mark Schiffman1

Division of Cancer Epidemiology and Genetics, National Cancer Institute, Bethesda,1 Digene Corporation, Gaithersburg,2 Information Management Services, Inc., Silver Spring, Maryland,5 Kaiser Permanente Center for Health Research, Portland, Oregon,3 Kaiser Foundation Research Institute, Oakland, California4

Received 13 August 2001/ Returned for modification 5 November 2001/ Accepted 14 December 2001

Reproducibility of the Hybrid Capture 2 Test (HC 2) for human papillomavirus (HPV) DNA detection was evaluated by assaying frozen cervical specimens in 1997 and again in 2001 from 1,775 women with normal cervical cytology. Using a cutoff point of 1.0 pg of HPV DNA/ml between a negative and a positive test result, the result of the kappa test for agreement was 0.72 (a kappa value of >0.60 is considered good agreement). Using cutoff points of 1.0 and 10.0 pg/ml between negative and low positive and between low positive and high positive, respectively, the kappa was 0.68 and the linear-weighted kappa was 0.76. The results of this study indicate that HC 2 testing is reproducible even among cytologically normal women with low test values.

* Corresponding author. Mailing address: Division of Cancer Epidemiology and Genetics, National Cancer Institute, 6120 Executive Plaza South, Room 7074, Rockville, MD 20892-7234. Phone: (301) 435-3976. Fax: (301) 402-0916. E-mail: pc95p{at}nih.gov.

Journal of Clinical Microbiology, March 2002, p. 1088-1090, Vol. 40, No. 3
0095-1137/02/$04.00+0     DOI: 10.1128/JCM.40.3.1088-1090.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

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