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Journal of Clinical Microbiology, March 2002, p. 811-816, Vol. 40, No. 3
0095-1137/02/$04.00+0     DOI: 10.1128/JCM.40.3.811-816.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.

Reproducibility of Bordetella pertussis Genomic DNA Fragments Generated by XbaI Restriction and Resolved by Pulsed-Field Gel Electrophoresis

Terri H. Hardwick,1 Brian Plikaytis,2 Pamela K. Cassiday,1 Gary Cage,3 Mark S. Peppler,4 Deborah Shea,5 David Boxrud,6 and Gary N. Sanden1*

Meningitis and Special Pathogens Branch,1 Biostatistics and Information Management Branch, Division of Bacterial and Mycotic Diseases, National Center for Infectious Diseases, Centers for Disease Control and Prevention, Atlanta, Georgia,2 Department of Clinical Microbiology, Arizona Department of Health Services, Phoenix, Arizona,3 Canada Department of Medical Microbiology and Immunology, Edmonton, Alberta, Canada,4 State Laboratory Institute, Diagnostic Laboratories, Massachusetts State Public Health Laboratory, Jamaica Plain, Massachusetts,5 Microbiology Laboratory, Minnesota Department of Health, Minneapolis, Minnesota6

Received 2 April 2001/ Returned for modification 19 June 2001/ Accepted 10 December 2001

The intra- and interlaboratory variabilities of the molecular size measurements of each DNA fragment contributing to three pulsed-field gel electrophoresis (PFGE) profiles were assessed, as were the reproducibilities of the entire PFGE profiles for three Bordetella pertussis strains. The major source of variability within a laboratory occurred between subcultures rather than within gels or between gels. Each PFGE profile was generated reproducibly and was objectively defined by the molecular sizes of its composite fragments. A strain or profile most suitable for use as an internal reference standard was identified.

* Corresponding author. Mailing address: Division of Bacterial and Mycotic Diseases, Centers for Disease Control and Prevention, Mailstop D-11, 1600 Clifton Rd., Atlanta, GA 30333. Phone: (404) 639-3024. Fax: (404) 639-4421. E-mail: gns1{at}cdc.gov.

Journal of Clinical Microbiology, March 2002, p. 811-816, Vol. 40, No. 3
0095-1137/02/$04.00+0     DOI: 10.1128/JCM.40.3.811-816.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.



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