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Journal of Clinical Microbiology, March 2002, p. 892-897, Vol. 40, No. 3
0095-1137/02/$04.00+0     DOI: 10.1128/JCM.40.3.892-897.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.

PCR Detection and Serological Evidence of Granulocytic Ehrlichial Infection in Roe Deer (Capreolus capreolus) and Chamois (Rupicapra rupicapra)

Jorge S. Liz,1* John W. Sumner,2 Kurt Pfister,1 and Michel Brossard1

Department of Immunology, Institute of Zoology, University of Neuchâtel, 2007 Neuchâtel, Switzerland,1 Viral and Rickettsial Zoonoses Branch, Division of Viral and Rickettsial Diseases, National Center for Infectious Diseases, Centers for Disease Control and Prevention, Atlanta, Georgia 303332

Received 6 August 2001/ Returned for modification 24 September 2001/ Accepted 4 December 2001

The role of wild mammals, such as roe deer (Capreolus capreolus) and chamois (Rupicapra rupicapra), in the epidemiology of granulocytic ehrlichiae in Switzerland was investigated. We tested blood samples for Ehrlichia phagocytophila genogroup 16S rRNA gene sequences by PCR and for immunoglobulin G antibodies against granulocytic ehrlichiae by indirect fluorescent-antibody assay (IFA). Overall means of 60.9% of 133 roe deer serum samples and 28.2% of 39 chamois serum samples were seroreactive by IFA. PCR results were positive for 18.4% of 103 roe deer serum samples as well. None of the 24 chamois blood samples tested were positive by PCR. Partial 16S rRNA gene and groESL heat shock operon sequences of three roe deer samples tested showed strong degrees of homology (>=99.7 and >=98.6%, respectively) with the sequences of granulocytic ehrlichiae isolated from humans. These results confirm that chamois, and particularly roe deer, are commonly infected with granulocytic ehrlichiae and provide evidence that these wild mammals are potential reservoirs for granulocytic ehrlichiae in Switzerland.

* Corresponding author. Mailing address: Department of Immunology, Institute of Zoology, University of Neuchâtel, Rue Emile-Argand 11, 2007 Neuchâtel, Switzerland. Phone: 41-32-7183016. Fax: 41-32-7183011. E-mail: jorge.liz{at}unine.ch.

Journal of Clinical Microbiology, March 2002, p. 892-897, Vol. 40, No. 3
0095-1137/02/$04.00+0     DOI: 10.1128/JCM.40.3.892-897.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.



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