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Journal of Clinical Microbiology, March 2002, p. 996-1000, Vol. 40, No. 3
0095-1137/02/$04.00+0     DOI: 10.1128/JCM.40.3.996-1000.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.

Detection of Heat-Stable Antigens of Campylobacter jejuni and C. coli by Direct Agglutination and Passive Hemagglutination

A. N. Oza,1,{dagger} R. T. Thwaites,1 D. R. A. Wareing,2 F. J. Bolton,2,{ddagger} and J. A. Frost1*

Campylobacter Reference Laboratory, Laboratory of Enteric Pathogens, Central Public Health Laboratory, London NW9 5HT,1 Preston Public Health Laboratory, Royal Preston Hospital, Preston, Lancashire PR2 9HG, England2

Received 30 July 2001/ Returned for modification 22 September 2001/ Accepted 6 January 2002

The two serotyping schemes for the detection of heat-stable antigens of Campylobacter jejuni and Campylobacter coli use the same strains for antiserum production but differ in the detection systems used for identifying agglutination. The Penner method uses passive hemagglutination (PHA) while the Laboratory of Enteric Pathogens method uses the same antisera but in a whole-bacterial-cell direct agglutination (DA) protocol. C. jejuni produces a polysaccharide capsule, which is antigenic, and is the main component detected by the PHA method. The DA method will detect both capsule antigens and lipopolysaccharide (LPS) or lipooligosaccharide (LOS) surface antigens. Comparison of both methods by using a selection of isolates from human infection has shown a range of variation in agglutination specificity, reflecting the differences in antigens detected by the two methods. While 27.4% of the 416 C. jejuni isolates reacted with the antisera raised against the same type strains by either method, the majority showed a range of more complex relationships. None of the 37 C. coli isolates reacted with the same antiserum by both methods. Together the two schemes gave a total of 102 distinct combined serogroups for C. jejuni and 16 for C. coli. Thus, while some clonally related isolates share the same capsule and LOS or LPS antigens, other strains appear to have a common capsule antigen but differ in their LPS or LOS structures or vice versa.

* Corresponding author. Mailing address: Campylobacter Reference Laboratory, Laboratory of Enteric Pathogens, Central Public Health Laboratory, 61 Colindale Ave., London NW9 5HT, United Kingdom. Phone: 44 208 200 4400, ext. 3772. Fax: 44 208 905 9929. E-mail: jafrost{at}phls.org.uk.

{dagger} Present address: Department of Veterinary Sciences, Queens University—Belfast, Stormont, Belfast BT4 3SD, United Kingdom.

{ddagger} Present address: Food Safety Microbiology Laboratory, Central Public Health Laboratory, London NW9 5HT, United Kingdom.

Journal of Clinical Microbiology, March 2002, p. 996-1000, Vol. 40, No. 3
0095-1137/02/$04.00+0     DOI: 10.1128/JCM.40.3.996-1000.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.



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