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Journal of Clinical Microbiology, April 2002, p. 1199-1206, Vol. 40, No. 4
0095-1137/02/$04.00+0     DOI: 10.1128/JCM.40.4.1199-1206.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.

Mitochondrial DNA Restriction Fragment Length Polymorphism (RFLP) and 18S Small-Subunit Ribosomal DNA PCR-RFLP Analyses of Acanthamoeba Isolated from Contact Lens Storage Cases of Residents in Southwestern Korea

Hyun-Hee Kong,¹ Ji-Yeol Shin,¹ Hak-Sun Yu,¹ Jin Kim,² Tae-Won Hahn,³ Young-Ho Hahn,⁴ and Dong-Il Chung^1*

Department of Parasitology, Kyungpook National University School of Medicine, Taegu,¹ Department of Parasitology, Seonam University College of Medicine, Namwon,² Department of Ophthalmology, Catholic University Medical College, Seoul,³ Department of Ophthalmology, Kosin University College of Medicine, Pusan, Korea⁴

Received 6 September 2001/ Returned for modification 8 October 2001/ Accepted 2 December 2001

We applied ribosomal DNA PCR-restriction fragment length polymorphism (RFLP) and mitochondrial DNA (mtDNA) RFLP analyses to 43 Acanthamoeba environmental isolates (KA/LH1 to KA/LH43) from contact lens storage cases in southwestern Korea. These isolates were compared to American Type Culture Collection strains and clinical isolates (KA/E1 to KA/E12) from patients with keratitis. Seven riboprint patterns were seen. To identify the species of the isolates, a phylogenetic tree was constructed based on the comparison of riboprint patterns with reference strains. Four types accounted for 39 of the isolates belonging to the A. castellanii complex. The most predominant (48.8%) type was A. castellanii KA/LH2 type, which had identical riboprint and mtDNA RFLP patterns to those of A. castellanii Castellani, KA/E3 and KA/E8. The riboprint and mtDNA RFLP patterns of the KA/LH7 (20.9%) type were identical to those of A. castellanii Ma, a corneal isolate from the United States. The riboprint and mtDNA RFLP patterns of the KA/LH1 (18.6%) type were the same as those of A. lugdunensis L3a, KA/E2, and KA/E12. The prevalent pattern for each type of Acanthamoeba in southwestern Korea was very different from that from southeastern Korea and Seoul, Korea. It is noteworthy that 38 (88.4%) out of 43 isolates from contact lens storage cases of the residents in southwestern Korea revealed mtDNA RFLP and riboprint patterns identical to those found for clinical isolates in our area. This indicates that most isolates from contact lens storage cases in the surveyed area are potential keratopathogens. More attention should be paid to the disinfection of contact lens storage cases to prevent possible amoebic keratitis.

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* Corresponding author. Mailing address: Department of Parasitology, Kyungpook National University School of Medicine, 101 Dongin-dong, Joong-ku, Taegu 700-422, Korea. Phone: 82-53-420-6956. Fax: 82-53-422-9330. E-mail: dichung{at}knu.ac.kr.

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Journal of Clinical Microbiology, April 2002, p. 1199-1206, Vol. 40, No. 4
0095-1137/02/$04.00+0     DOI: 10.1128/JCM.40.4.1199-1206.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.

This article has been cited by other articles:

• Yu, H.-S., Kong, H.-H., Kim, S.-Y., Hahn, Y.-H., Hahn, T.-W., Chung, D.-I. (2004). Laboratory Investigation of Acanthamoeba lugdunensis from Patients with Keratitis. IOVS 45: 1418-1426 [Abstract] [Full Text]