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Journal of Clinical Microbiology, April 2002, p. 1271-1276, Vol. 40, No. 4
0095-1137/02/$04.00+0     DOI: 10.1128/JCM.40.4.1271-1276.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.

Simultaneous Differentiation and Typing of Entamoeba histolytica and Entamoeba dispar

Mehreen Zaki, Parool Meelu,,{dagger} Wei Sun,,{ddagger} and C. Graham Clark*

Department of Infectious and Tropical Diseases, London School of Hygiene and Tropical Medicine, London WC1E 7HT, United Kingdom

Received 17 October 2001/ Returned for modification 13 December 2001/ Accepted 20 January 2002

Sequences corresponding to some of the polymorphic loci previously reported from Entamoeba histolytica have been detected in Entamoeba dispar. Comparison of nucleotide sequences of two loci between E. dispar strain SAW760 and E. histolytica strain HM-1:IMSS revealed significant differences in both repeat and flanking regions. The tandem repeat units varied not only in sequence but also in number and arrangement between the two species at both the loci. Using the sequences obtained, primer pairs aimed at amplifying species-specific products were designed and tested on a variety of E. histolytica and E. dispar samples. Amplification results were in complete agreement with the original species classification in all cases, and the PCR products displayed discernible size and pattern variations among the isolates.

* Corresponding author. Mailing address: Department of Infectious and Tropical Diseases, London School of Hygiene and Tropical Medicine, Keppel St., London WC1E 7HT, United Kingdom. Phone: 44-207-927 2351. Fax: 44-207-636 8739. E-mail: graham.clark{at}lshtm.ac.uk.

{dagger} Present address: Department of Pediatric Hematology/Oncology, Medical School Hannover, D-30625 Hannover, Germany.

{ddagger} Present address: Kirin Pharmaceuticals (China) Co., Ltd., Beijing, China.

Journal of Clinical Microbiology, April 2002, p. 1271-1276, Vol. 40, No. 4
0095-1137/02/$04.00+0     DOI: 10.1128/JCM.40.4.1271-1276.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.



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