This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Nosek, J.
Right arrow Articles by Fukuhara, H.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Nosek, J.
Right arrow Articles by Fukuhara, H.

 Previous Article  |  Next Article 

Journal of Clinical Microbiology, April 2002, p. 1283-1289, Vol. 40, No. 4
0095-1137/02/$04.00+0     DOI: 10.1128/JCM.40.4.1283-1289.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.

Mitochondrial Telomeres as Molecular Markers for Identification of the Opportunistic Yeast Pathogen Candida parapsilosis

Jozef Nosek,1* L'ubomír Tomáska,2 Adriana Rycovská,1 and Hiroshi Fukuhara3

Departments of Biochemistry,1 Genetics, Faculty of Natural Sciences, Comenius University, 84215 Bratislava, Slovakia,2 Institut Curie, Section de Recherche, Centre Universitaire Paris XI, 91405 Orsay, France3

Received 25 July 2001/ Returned for modification 14 December 2001/ Accepted 24 January 2002

Recent studies have demonstrated that a large number of organisms carry linear mitochondrial DNA molecules possessing specialized telomeric structures at their ends. Based on this specific structural feature of linear mitochondrial genomes, we have developed an approach for identification of the opportunistic yeast pathogen Candida parapsilosis. The strategy for identification of C. parapsilosis strains is based on PCR amplification of specific DNA sequences derived from the mitochondrial telomere region. This assay is complemented by immunodetection of a protein component of mitochondrial telomeres. The results demonstrate that mitochondrial telomeres represent specific molecular markers with potential applications in yeast diagnostics and taxonomy.

* Corresponding author. Mailing address: Department of Biochemistry, Faculty of Natural Sciences, Comenius University, Mlynská dolina CH-1, 84215 Bratislava, Slovakia. Phone: 421.2.60296.536. Fax: 421.2.60296.452. E-mail: nosek{at}fns.uniba.sk.

Journal of Clinical Microbiology, April 2002, p. 1283-1289, Vol. 40, No. 4
0095-1137/02/$04.00+0     DOI: 10.1128/JCM.40.4.1283-1289.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Silva, A. P., Miranda, I. M., Lisboa, C., Pina-Vaz, C., Rodrigues, A. G. (2009). Prevalence, Distribution, and Antifungal Susceptibility Profiles of Candida parapsilosis, C. orthopsilosis, and C. metapsilosis in a Tertiary Care Hospital. J. Clin. Microbiol. 47: 2392-2397 [Abstract] [Full Text]  
  • Borman, A. M., Linton, C. J., Oliver, D., Palmer, M. D., Szekely, A., Odds, F. C., Johnson, E. M. (2009). Pyrosequencing Analysis of 20 Nucleotides of Internal Transcribed Spacer 2 Discriminates Candida parapsilosis, Candida metapsilosis, and Candida orthopsilosis. J. Clin. Microbiol. 47: 2307-2310 [Abstract] [Full Text]  
  • Tay, S. T., Na, S. L., Chong, J. (2009). Molecular differentiation and antifungal susceptibilities of Candida parapsilosis isolated from patients with bloodstream infections. J Med Microbiol 58: 185-191 [Abstract] [Full Text]  
  • Trofa, D., Gacser, A., Nosanchuk, J. D. (2008). Candida parapsilosis, an Emerging Fungal Pathogen. Clin. Microbiol. Rev. 21: 606-625 [Abstract] [Full Text]  
  • Kocsube, S., Toth, M., Vagvolgyi, C., Doczi, I., Pesti, M., Pocsi, I., Szabo, J., Varga, J. (2007). Occurrence and genetic variability of Candida parapsilosis sensu lato in Hungary. J Med Microbiol 56: 190-195 [Abstract] [Full Text]  
  • Lasker, B. A., Butler, G., Lott, T. J. (2006). Molecular Genotyping of Candida parapsilosis Group I Clinical Isolates by Analysis of Polymorphic Microsatellite Markers.. J. Clin. Microbiol. 44: 750-759 [Abstract] [Full Text]  
  • Tavanti, A., Davidson, A. D., Gow, N. A. R., Maiden, M. C. J., Odds, F. C. (2005). Candida orthopsilosis and Candida metapsilosis spp. nov. To Replace Candida parapsilosis Groups II and III. J. Clin. Microbiol. 43: 284-292 [Abstract] [Full Text]  
  • Rycovska, A., Valach, M., Tomaska, L., Bolotin-Fukuhara, M., Nosek, J. (2004). Linear versus circular mitochondrial genomes: intraspecies variability of mitochondrial genome architecture in Candida parapsilosis. Microbiology 150: 1571-1580 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»