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Journal of Clinical Microbiology, May 2002, p. 1626-1635, Vol. 40, No. 5
0095-1137/02/$04.00+0     DOI: 10.1128/JCM.40.5.1626-1635.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.

Multilocus Sequence Typing for Characterization of Clinical and Environmental Salmonella Strains

Mamuka Kotetishvili,1,2 O. Colin Stine,1 Arnold Kreger,1 J. Glenn Morris, Jr.,1 and Alexander Sulakvelidze1*

Division of Molecular Epidemiology, Department of Epidemiology and Preventive Medicine, University of Maryland School of Medicine, Baltimore, Maryland 21201,1 Kanchaveli Institute of Plant Protection, Tbilisi, Georgia 3800772

Received 17 October 2001/ Returned for modification 29 January 2002/ Accepted 12 February 2002

Multilocus sequence typing (MLST) based on the 16S RNA, pduF, glnA, and manB genes was developed for Salmonella, and its discriminatory ability was compared to those of pulsed-field gel electrophoresis (PFGE) and serotyping. PFGE differentiated several strains undifferentiable by serotyping, and 78 distinct PFGE types were identified among 231 Salmonella isolates grouped into 22 serotypes and 12 strains of undetermined serotype. The strains of several PFGE types were further differentiated by MLST, which suggests that the discriminatory ability of MLST for the typing of Salmonella is better than that of serotyping and/or PFGE typing. manB-based sequence typing identified two distinct genetic clusters containing 32 of 54 (59%) clinical isolates whose manB gene sequences were analyzed. The G+C contents and Splitstree analysis of the manB, glnA, and pduF genes of Salmonella indicated that the genes differ in their evolutionary origins and that recombination played a significant role in their evolution.

* Corresponding author. Mailing address: Department of Epidemiology and Preventive Medicine, University of Maryland School of Medicine, MSTF Bldg., Room 9-34D, 10 South Pine St., Baltimore, MD 21201. Phone: (410) 706-4587. Fax: (410) 706-4581. E-mail: asulakve{at}epi.umaryland.edu.

Journal of Clinical Microbiology, May 2002, p. 1626-1635, Vol. 40, No. 5
0095-1137/02/$04.00+0     DOI: 10.1128/JCM.40.5.1626-1635.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.



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