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Journal of Clinical Microbiology, May 2002, p. 1715-1718, Vol. 40, No. 5
0095-1137/02/$04.00+0     DOI: 10.1128/JCM.40.5.1715-1718.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.

Evaluation of an Immunofluorescent-Antibody Test Using Monoclonal Antibodies Directed against Enterocytozoon bieneusi and Encephalitozoon intestinalis for Diagnosis of Intestinal Microsporidiosis in Bamako (Mali)

O. Alfa Cisse,1 A. Ouattara,2 M. Thellier,1 I. Accoceberry,3 S. Biligui,1 D. Minta,2 O. Doumbo,2 I. Desportes-Livage,1 M. A. Thera,2 M. Danis,1 and A. Datry1*

Unité INSERM 511, Laboratoire de Parasitologie-Mycologie, Centre Hospitalier-Universitaire de la Pitié-Salpêtrière, 75013 Paris,1 Laboratoire de Parasitologie-Mycologie, Centre Hospitalier-Universitaire de Saint-André, 33000 Bordeaux, France,3 Département d'Epidémiologie des Affections Parasitaires, Faculté de Médecine, Pharmacie, et d'Odonto-Stomatologie, Bamako, Mali2

Received 5 October 2001/ Returned for modification 4 December 2001/ Accepted 22 February 2002

A 2-month study was carried out in Mali to evaluate an immunofluorescent-antibody test (IFAT) using monoclonal probes specific for Enterocytozoon bieneusi or Encephalitozoon intestinalis. Sixty-one human immunodeficiency virus (HIV)-seropositive adult patients and 71 immunocompetent children were enrolled. Microsporidia were detected in stools from 8 of 61 patients (13.1%) seropositive for HIV. A single species, E. bieneusi, was identified. All the children were negative for microsporidia. The sensitivity and specificity of IFAT were 100% compared with those of PCR, which was used as the "gold standard." Moreover, species identification by IFAT was more rapid and less expensive than that by PCR. These results show the suitability of IFAT for detection of microsporidia in developing countries.

* Corresponding author. Mailing address: Laboratoire de Parasitologie-Mycologie—INSERM 511, CHU Pitié-Salpêtrière, 47, Boulevard de l'Hôpital, 75013 Paris, France. Phone: 33 1-42 16 01 28. Fax: 33 1-42 16 01 65. E-mail: annick.datry{at}psl.ap-hop-paris.fr.

Journal of Clinical Microbiology, May 2002, p. 1715-1718, Vol. 40, No. 5
0095-1137/02/$04.00+0     DOI: 10.1128/JCM.40.5.1715-1718.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.



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