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Journal of Clinical Microbiology, May 2002, p. 1826-1830, Vol. 40, No. 5
0095-1137/02/$04.00+0     DOI: 10.1128/JCM.40.5.1826-1830.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.

Sequence Analysis of the Ribosomal DNA Intergenic Spacer 1 Regions of Trichosporon Species

Takashi Sugita,1* Masamitsu Nakajima,2,{dagger} Reiko Ikeda,1 Toshiharu Matsushima,2 and Takako Shinoda1

Department of Microbiology, Meiji Pharmaceutical University, Kiyose, Tokyo,1 Division of Respiratory Diseases, Department of Medicine, Kawasaki Medical School, Kurashiki, Okayama, Japan2

Received 15 October 2001/ Returned for modification 18 December 2001/ Accepted 3 March 2002

We determined the sequence of the intergenic spacer (IGS) 1 region, which is located between the 26S and 5S rRNA genes, in 25 species of the genus Trichosporon. IGS 1 sequences varied in length from 195 to 719 bp. Comparative sequence analysis suggested that the divergence of IGS 1 sequences has been greater than that of the internal transcribed spacer regions. We also identified five genotypes of T. asahii, which is a major causative agent of deep-seated trichosporonosis, based on the IGS 1 sequences of 43 strains. Most of the isolates that originated in Japan were of genotype 1, whereas the American isolates were of genotype 3 or 5. Our results suggest that analysis of IGS regions provides a powerful method to distinguish between phylogenetically closely related species and that a geographic substructure may exist among T. asahii clinical isolates.

* Corresponding author. Mailing address: Department of Microbiology, Meiji Pharmaceutical University, 2-522-1 Noshio, Kiyose, Tokyo 204-8588, Japan. Phone: 81-424-95-8762. Fax: 81-424-95-8762. E-mail: sugita{at}my-pharm.ac.jp.

{dagger} Present address: Second Department of Internal Medicine, Hiroshima University School of Medicine, Hiroshima, Japan.

Journal of Clinical Microbiology, May 2002, p. 1826-1830, Vol. 40, No. 5
0095-1137/02/$04.00+0     DOI: 10.1128/JCM.40.5.1826-1830.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.



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