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Journal of Clinical Microbiology, May 2002, p. 1848-1850, Vol. 40, No. 5
0095-1137/02/$04.00+0     DOI: 10.1128/JCM.40.5.1848-1850.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.

Detection of Porcine Circovirus Types 1 and 2 in Serum and Tissue Samples of Pigs with and without Postweaning Multisystemic Wasting Syndrome

Maria Calsamiglia,1* Joaquim Segalés,1,2 Josefina Quintana,2 Carles Rosell,2 and Mariano Domingo1,2

Centre de Recerca en Sanitat Animal (CReSA), Campus Universitari Bellaterra,1 Departament de Sanitat i Anatomia Animals, Facultat de Veterinària, Universitat Autònoma de Barcelona, 08193 Bellaterra, Spain2

Received 11 October 2001/ Returned for modification 31 December 2001/ Accepted 8 February 2002

Presence of porcine circovirus type 1 (PCV1) and PCV2 was studied in sera and superficial inguinal lymph nodes from postweaning multisystemic wasting syndrome (PMWS)-affected and non-PMWS-affected pigs by using in situ hybridization and PCR. PCV1 and PCV2 were found in less than 3% and more than 50% of the samples, respectively. The most sensitive technique and site was PCR in superficial inguinal lymph nodes, but in situ hybridization correlated better with presence of characteristic lesions.

* Corresponding author. Mailing address: Unitat d'Anatomia Patològica, Facultat de Veterinària, Universitat Autònoma de Barcelona, 08193 Bellaterra, Barcelona, Spain. Phone: (34) 93 581 31 45. Fax: (34) 93 581 31 42. E-mail: Maria.Calsamiglia{at}uab.es.

Journal of Clinical Microbiology, May 2002, p. 1848-1850, Vol. 40, No. 5
0095-1137/02/$04.00+0     DOI: 10.1128/JCM.40.5.1848-1850.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.



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