This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Gounder, C.
Right arrow Articles by Dorman, S. E.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Gounder, C.
Right arrow Articles by Dorman, S. E.

 Previous Article  |  Next Article 

Journal of Clinical Microbiology, June 2002, p. 1989-1993, Vol. 40, No. 6
0095-1137/02/$04.00+0     DOI: 10.1128/JCM.40.6.1989-1993.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.

Field Evaluation of a Rapid Immunochromatographic Test for Tuberculosis

Celine Gounder,1 Fernanda Carvalho de Queiroz Mello,2 Marcus B. Conde,2 William R. Bishai,1,3 Afrânio L. Kritski,2 Richard E. Chaisson,1,3 and Susan E. Dorman3*

Johns Hopkins University School of Hygiene and Public Health,1 Johns Hopkins University School of Medicine, Baltimore, Maryland,3 Unidade de Pesquisa em Tuberculose, Hospital Universitario Clementino Fraga Filho, Universidade Federal do Rio de Janeiro, Rio de Janeiro, Brazil2

Received 15 November 2001/ Returned for modification 17 January 2002/ Accepted 13 March 2002

Rapid diagnostic tests for tuberculosis (TB) are needed to facilitate early treatment of TB and prevention of Mycobacterium tuberculosis transmission. The ICT Tuberculosis test is a rapid, card-based immunochromatographic test for detection of antibodies directed against M. tuberculosis antigens. The objective of the study was to evaluate the performance of the ICT Tuberculosis test for the diagnosis of active pulmonary TB (PTB) with whole blood, plasma, and serum from patients suspected of having PTB and from asymptomatic controls in a setting with a high prevalence of PTB. Seventy patients suspected of having PTB (and who were later confirmed to have or not to have PTB by use of M. tuberculosis culture as the "gold standard") and 42 controls were studied. Twenty-one controls were neither vaccinated with Mycobacterium bovis bacillus Calmette-Guérin (BCG) nor tuberculin skin test (TST) positive (group A controls), and 21 controls were TST positive and/or had previously been vaccinated with BCG (group B controls). Study subjects were drawn from one hospital and one primary health care unit in Rio de Janeiro City, Brazil. One version of the test (ICT-1) was evaluated by using whole blood, plasma, and serum samples. Sera obtained for this study were frozen and later tested with a manufacturer-modified version of the test (ICT-2). Among the patients suspected of having PTB, the sensitivities of the ICT-1 with whole blood, serum, and plasma were 83, 65, and 70%, respectively, and the specificities were 46, 67, and 56%, respectively. Among the group A controls, the specificities of ICT-1 with the three specimen types were 95, 100, and 95%, respectively. Among the group B controls, the specificities of ICT-1 with the three specimen types were 71, 86, and 86%, respectively. Among the patients suspected of having PTB, the sensitivity of ICT-2 was 70% and the specificity was 65%. Among the group A controls, the specificity of ICT-2 was 95%, and among the group B controls, the specificity of ICT-2 was 81%. With a 29% observed prevalence of PTB among patients suspected of having PTB, the positive predictive values of the ICT tests ranged from 39 to 50% and the negative predictive values ranged from 82 to 87%. The ICT Tuberculosis tests were not sufficiently predictive to warrant their widespread use as routine diagnostic tests for PTB in this setting. However, further evaluation of these tests in specific epidemiologic settings may be warranted.

* Corresponding author. Mailing address: Center for Tuberculosis Research, Johns Hopkins University, 424 North Bond St., Baltimore, MD 21231. Phone: (410) 955-1755. Fax: (410) 955-0740. E-mail: dsusan1{at}jhmi.edu.

Journal of Clinical Microbiology, June 2002, p. 1989-1993, Vol. 40, No. 6
0095-1137/02/$04.00+0     DOI: 10.1128/JCM.40.6.1989-1993.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Kassa-Kelembho, E., Kassa, E., Zandanga, G., Service, Y.-B., Ignaleamoko, A., Talarmin, A. (2006). Poor Performance of a Novel Serological Test for Diagnosis of Pulmonary Tuberculosis in Bangui, Central African Republic. CVI 13: 702-703 [Abstract] [Full Text]  
  • Palomino, J. C. (2005). Nonconventional and new methods in the diagnosis of tuberculosis: feasibility and applicability in the field. Eur Respir J 26: 339-350 [Abstract] [Full Text]  
  • Tiwari, R. P., Tiwari, D., Garg, S. K., Chandra, R., Bisen, P. S. (2005). Glycolipids of Mycobacterium tuberculosis Strain H37Rv Are Potential Serological Markers for Diagnosis of Active Tuberculosis. CVI 12: 465-473 [Abstract] [Full Text]  
  • Imaz, M. S., Comini, M. A., Zerbini, E., Sequeira, M. D., Latini, O., Claus, J. D., Singh, M. (2004). Evaluation of Commercial Enzyme-Linked Immunosorbent Assay Kits for Detection of Tuberculosis in Argentinean Population. J. Clin. Microbiol. 42: 884-887 [Abstract] [Full Text]  
  • Conde, M. B., Suffys, P., Lapa e Silva, J. R., Kritski, A. L., Dorman, S. E. (2004). Immunoglobulin A (IgA) and IgG Immune Responses against P-90 Antigen for Diagnosis of Pulmonary Tuberculosis and Screening for Mycobacterium tuberculosis Infection. CVI 11: 94-97 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»