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Journal of Clinical Microbiology, June 2002, p. 2231-2233, Vol. 40, No. 6
0095-1137/02/$04.00+0     DOI: 10.1128/JCM.40.6.2231-2233.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.

Differentiation of Aspergillus ustus Strains by Random Amplification of Polymorphic DNA

Peter-Michael Rath,1* Katrin Petermeier,1 Paul E. Verweij,2 and Rainer Ansorg1

Institut für Medizinische Mikrobiologie, Universitätsklinikum Essen, Essen, Germany,1 Department of Medical Microbiology, University Medical Center, Nijmegen, The Netherlands2

Received 21 November 2001/ Returned for modification 18 February 2002/ Accepted 28 March 2002

The sodium dodecyl sulfate-polyacrylamide gel electrophoresis patterns of water-soluble proteins and the randomly amplified polymorphic DNA (RAPD) patterns of whole-cell lysates from 21 Aspergillus ustus isolates, including 11 reference strains and 10 patient and environmental strains from one hospital, were investigated. All isolates showed identical protein patterns. The RAPD assay discriminated between all reference strains. Comparison of hospital isolates showed identical RAPD patterns in some of the patient and environmental isolates. The data indicate that the RAPD technique is useful for fingerprinting A. ustus.

* Corresponding author. Mailing address: Institut für Medizinische Mikrobiologie, Universitätsklinikum Essen, D-45122 Essen, Germany. Phone: (49) 201-723 3538. Fax: (49) 201-723 5729. E-mail: rath{at}uni-essen.de.

Journal of Clinical Microbiology, June 2002, p. 2231-2233, Vol. 40, No. 6
0095-1137/02/$04.00+0     DOI: 10.1128/JCM.40.6.2231-2233.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.



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