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Journal of Clinical Microbiology, June 2002, p. 2257-2259, Vol. 40, No. 6
0095-1137/02/$04.00+0     DOI: 10.1128/JCM.40.6.2257-2259.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.

Comparative Study of Two Methods for RNA Extraction Prior to Detection of Resistance to Human Immunodeficiency Virus Type 1 with the Line Probe Assay

Jose María Eiros,1* Cristina Labayru,2 Beatriz Hernández,1 Raúl Ortiz de Lejarazu,1 and Antonio Rodríguez Torres1

Microbiology Service, University Hospital of Valladolid,1 Microbiology Service, University Hospital "Rio Hortega," Valladolid, Spain2

Received 1 November 2001/ Returned for modification 5 January 2002/ Accepted 26 March 2002

We evaluated two methods from Roche and Promega for RNA extraction prior to the genotypic detection of human immunodeficiency virus type 1 resistance by line probe assay (LiPA). Fifty plasma RNA extracts were processed in parallel by LiPA. Results obtained by the Roche method were superior in the proportion of amplified samples, the percentage of mutated samples, and band intensity.

* Corresponding author. Mailing address: Microbiology, Sixth Floor, Faculty of Medicine, Avda. Ramón y Cajal s/n, 47005 Valladolid, Spain. Phone: 34-983 423063. Fax: 34-983 423066. E-mail: eiros{at}med.uva.es.

Journal of Clinical Microbiology, June 2002, p. 2257-2259, Vol. 40, No. 6
0095-1137/02/$04.00+0     DOI: 10.1128/JCM.40.6.2257-2259.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.





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