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Journal of Clinical Microbiology, June 2002, p. 2273-2274, Vol. 40, No. 6
0095-1137/02/$04.00+0     DOI: 10.1128/JCM.40.6.2273-2274.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.

Quantitative Detection of Respiratory Chlamydia pneumoniae Infection by Real-Time PCR

Yvonne Kuoppa,* Jens Boman, Lena Scott,,{dagger} Urban Kumlin, Iréne Eriksson, and Annika Allard

Department of Virology, University of Umeå, Umeå, Sweden

Received 16 July 2001/ Returned for modification 11 January 2002/ Accepted 27 March 2002

Real-time PCR was evaluated as a quantitative diagnostic method for Chlamydia pneumoniae infection using different respiratory samples. Real-time PCR had efficiency equal to or better than that of nested touchdown PCR. This study confirmed sputum as the best sampling material to detect an ongoing C. pneumoniae infection.

* Corresponding author. Mailing address: Department of Virology, University of Umeå, S-901 85 Umeå, Sweden. Phone: (46) 90-785 8449. Fax: (46) 90-12 99 05. E-mail: Yvonne.Kuoppa{at}climi.umu.se.

{dagger} Present address: Department of Woman and Child Health, Karolinska Institutet, Astrid Lindgrens Children's Hospital, S-171 76 Stockholm, Sweden.

Journal of Clinical Microbiology, June 2002, p. 2273-2274, Vol. 40, No. 6
0095-1137/02/$04.00+0     DOI: 10.1128/JCM.40.6.2273-2274.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.



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