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Journal of Clinical Microbiology, June 2002, p. 2285-2287, Vol. 40, No. 6
0095-1137/02/$04.00+0     DOI: 10.1128/JCM.40.6.2285-2287.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.

High Variability between Results of Different In-House Tests for Cytomegalovirus (CMV) Monitoring and a Standardized Quantitative Plasma CMV PCR Assay

Lutz von Müller,1 Walter Hampl,1 Joachim Hinz,1 Helga Meisel,2 Angela Reip,2 Elisabeth Engelmann,3 Regine Heilbronn,3 Barbara Gärtner,4 Oliver Krämer,4 Hermann Einsele,5 Holger Hebart,5 Tatjana Ljubicic,5 Jürgen Löffler,5 and Thomas Mertens1*

Abteilung Virologie, Universität Ulm, Ulm,1 Institut für Virologie, Charité Berlin,2 Institut für Virologie, Freie Universität Berlin, Berlin,3 Abteilung für Virologie, Universität Homburg, Homburg,4 Medizinische Klinik und Poliklinik, Abteilung II, Universität Tübingen, Tübingen, Germany5

Received 15 October 2001/ Accepted 23 March 2002

A total of 2,718 blood samples were analyzed in five virological laboratories for the presence of cytomegalovirus (CMV) by in-house tests and one standardized plasma PCR assay. Results from in-house tests showed remarkable variability. Detection of CMV pp65 antigen or DNA from cells was more sensitive than that by plasma CMV PCR assay.

* Corresponding author. Mailing address: Abteilung Virologie, Universitätsklinikum Ulm, Albert Einstein Allee 11, 89081 Ulm, Germany. Phone: 49-731-50023341. Fax: 49-731-50023337. E-mail: thomas.mertens{at}medizin.uni-ulm.de.

Journal of Clinical Microbiology, June 2002, p. 2285-2287, Vol. 40, No. 6
0095-1137/02/$04.00+0     DOI: 10.1128/JCM.40.6.2285-2287.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.



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