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Journal of Clinical Microbiology, June 2002, p. 2291-2296, Vol. 40, No. 6
0095-1137/02/$04.00+0 DOI: 10.1128/JCM.40.6.2291-2296.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.
Expression of the Bundle-Forming Pilus by Enteropathogenic Escherichia coli Strains of Heterologous Serotypes
Juana Gismero-Ordoñez,1 Monique Dall'Agnol,2 Luiz R. Trabulsi,1 and Jorge A. Girón2*
Departamento de Microbiologia, Universidade de São Paulo, and Instituto Butantã, São Paulo, Brazil,1
Centro de Investigaciones en Ciencias Microbiológicas, Instituto de Ciencias, Benemérita Universidad Autónoma de Puebla, Puebla, Mexico2
Received 17 December 2001/
Returned for modification 27 January 2002/
Accepted 1 March 2002
The production of the bundle-forming pilus subunit BfpA was investigated in 44 enteropathogenic Escherichia coli (EPEC) diarrheal isolates after growth on several conventional bacteriological media. In all, the use of brucella agar supplemented with 4.5 mM sodium bisulfite resulted in a higher detection of BfpA-producing EPEC.
* Corresponding author. Mailing address: Centro de Investigaciones en Ciencias Microbiológicas, Instituto de Ciencias, Benemérita Universidad Autónoma de Puebla, Edificio 76, Complejo de Ciencias, Ciudad Universitaria, C. P. 72000, Puebla, Pue, Mexico. Phone: 52 222 332010. Fax: 52 222 444518. E-mail:
jagiron{at}yahoo.com.
Journal of Clinical Microbiology, June 2002, p. 2291-2296, Vol. 40, No. 6
0095-1137/02/$04.00+0 DOI: 10.1128/JCM.40.6.2291-2296.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.
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