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Journal of Clinical Microbiology, July 2002, p. 2452-2458, Vol. 40, No. 7
0095-1137/02/$04.00+0     DOI: 10.1128/JCM.40.7.2452-2458.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.

Clostridium difficile Genotyping Based on slpA Variable Region in S-Layer Gene Sequence: an Alternative to Serotyping

Tuomo Karjalainen,1 Nicolas Saumier,1 Marie-Claude Barc,1 Michel Delmée,2 and Anne Collignon1*

Université de Paris-Sud, Faculté de Pharmacie, Département de Microbiologie, 92296 ChÂtenay-Malabry Cedex, France,1 Université Catholique de Louvain, Faculté de Médecine, Unité de Microbiologie, Brussels 1200, Belgium2

Received 10 January 2002/ Returned for modification 16 February 2002/ Accepted 13 April 2002

Recent investigations of Clostridium difficile cell wall components have revealed the presence of an S-layer encoded by the slpA gene. The aim of this study was to determine whether slpA genotyping can be used as an alternative to serotyping. The variable regions of slpA were amplified by PCR from serogroup reference strains and various clinical isolates chosen randomly. Amplified products were analyzed after restriction enzyme digestion and DNA sequencing. The sequences of the variable region of the SlpA protein were found to be strictly identical within a given serogroup but divergent between serogroups. These preliminary results suggest that PCR-restriction fragment length polymorphism, in conjunction with DNA sequencing of the slpA variable region, could constitute an alternative typing method for determining C. difficile serotypes.

* Corresponding author. Mailing address: Université de Paris-Sud, Faculté de Pharmacie, Département de Microbiologie, 5 rue JB Clement, 92296 ChÂtenay-Malabry Cedex, France. Phone: (33) 1 46 83 55 49. Fax: (33) 1 46 83 55 37. E-mail: anne.collignon{at}cep.u-psud.fr.

Journal of Clinical Microbiology, July 2002, p. 2452-2458, Vol. 40, No. 7
0095-1137/02/$04.00+0     DOI: 10.1128/JCM.40.7.2452-2458.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.



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