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Journal of Clinical Microbiology, July 2002, p. 2472-2475, Vol. 40, No. 7
0095-1137/02/$04.00+0     DOI: 10.1128/JCM.40.7.2472-2475.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.

Comparison of an Assay Using Signal Amplification of the Heat-Dissociated p24 Antigen with the Roche Monitor Human Immunodeficiency Virus RNA Assay

Alvaro Pascual,1 Ada Cachafeiro,2 Michele L. Funk,3 and Susan A. Fiscus2*

Department of Microbiology, Universidad de Sevilla, Seville, Spain,1 Department of Microbiology & Immunology,2 Department of Epidemiology, University of North Carolina, Chapel Hill, North Carolina3

Received 21 December 2001/ Returned for modification 15 February 2002/ Accepted 23 March 2002

We compared an assay using signal amplification of a heat-dissociated p24 antigen (HDAg) with the Roche Monitor human immunodeficiency virus (HIV) RNA assay. The two assays gave comparable results when 130 specimens from 130 patients were tested (r = 0.60, P < 0.0001). The HDAg assay was almost as sensitive (85%) as the Roche HIV RNA kit (95%), just as specific (25 negative results from 25 HIV seronegative volunteers [100%]), less variable (mean log standard deviation of 0.07 compared to 0.11 when eight specimens were tested three or four times), and less expensive (reagent and labor costs, $8 versus $75). The assay appeared to be useful for monitoring established patients (n = 17) and identifying seroconverters (n = 4). HIV subtypes A to F were all recognized. This assay should be useful for monitoring patients in resource-poor countries and for monitoring vaccine recipients.

* Corresponding author. Mailing address: Department of Microbiology & Immunology, University of North Carolina at Chapel Hill, Chapel Hill, NC 27599-7140. Phone: (919) 966-6872. Fax: (919) 966-9873. E-mail: fiscussa{at}med.unc.edu.

Journal of Clinical Microbiology, July 2002, p. 2472-2475, Vol. 40, No. 7
0095-1137/02/$04.00+0     DOI: 10.1128/JCM.40.7.2472-2475.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.



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