This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Talukder, K. A.
Right arrow Articles by Sack, D. A.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Talukder, K. A.
Right arrow Articles by Sack, D. A.

 Previous Article  |  Next Article 

Journal of Clinical Microbiology, July 2002, p. 2490-2497, Vol. 40, No. 7
0095-1137/02/$04.00+0     DOI: 10.1128/JCM.40.7.2490-2497.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.

Phenotypic and Genotypic Characterization of Serologically Atypical Strains of Shigella flexneri Type 4 Isolated in Dhaka, Bangladesh

Kaisar A. Talukder,* M. Aminul Islam, Dilip K. Dutta, Ferdaus Hassan, Ashrafus Safa, G. B. Nair, and David A. Sack

International Centre for Diarrhoeal Disease Research, Bangladesh, Dhaka-1000, Bangladesh

Received 3 December 2001/ Returned for modification 19 March 2002/ Accepted 21 April 2002

Twenty-one atypical Shigella flexneri type 4 strains isolated from patients attending the Dhaka treatment center of the International Centre for Diarrhoeal Disease Research, Bangladesh, were extensively characterized and compared with S. flexneri serotypes 4a and 4b. The atypical strains agglutinated only with the type antigen factor 4 and did not agglutinate with any group factors, thereby excluding their characterization into serotype 4a or 4b. Of the 21 strains, 85.7% did not ferment mannitol but were able to ferment most of the sugars, whereas the remaining 14.3% strains fermented mannitol but were unable to ferment most of the sugars. Most of the strains were resistant to ampicillin, tetracycline, and trimethoprim-sulfomethoxazole. All of the strains harbored the 140-MDa plasmid, had the ipaH gene, had the sen gene (encoding Shigella enterotoxin 2), had the ability to bind Congo red, and were positive for keratoconjunctivitis in the guinea pig eye, attesting their invasive properties. All of the strains contained a middle-range plasmid (35 to 62 MDa) as well as a number of stable small plasmids, yielding mainly two plasmid profiles which were different from those of 4a and 4b strains. Conjugation and curing experiments suggested that the middle-range plasmids harbored a self-transferable multiple antibiotic resistance marker. Pulsed-field gel electrophoresis analysis of all of the tested strains yielded two types with numerous subtypes, whereas ribotyping yielded only two types which were completely different from those of types 4a and 4b. This study concluded that two different clones of atypical S. flexneri type 4 exist and strongly suggests that these are new subserotypes of S. flexneri that await further serological classification.

* Corresponding author. Mailing address: Enteric Bacteriology Laboratory, Laboratory Sciences Division, International Centre for Diarrhoeal Disease Research, Bangladesh, GPO Box 128, Dhaka-1000, Bangladesh. Phone: 880 2 8811751-60. Fax: 880 2 8812529. E-mail: kaisar{at}icddrb.org.

Journal of Clinical Microbiology, July 2002, p. 2490-2497, Vol. 40, No. 7
0095-1137/02/$04.00+0     DOI: 10.1128/JCM.40.7.2490-2497.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Talukder, K. A., Aslam, M., Islam, Z., Azmi, I. J., Dutta, D. K., Hossain, S., Nur-E-Kamal, A., Nair, G. B., Cravioto, A., Sack, D. A., Endtz, H. P. (2008). Prevalence of Virulence Genes and Cytolethal Distending Toxin Production in Campylobacter jejuni Isolates from Diarrheal Patients in Bangladesh. J. Clin. Microbiol. 46: 1485-1488 [Abstract] [Full Text]  
  • Grimont, F., Lejay-Collin, M., Talukder, K. A., Carle, I., Issenhuth, S., Le Roux, K., Grimont, P. A. D. (2007). Identification of a group of shigella-like isolates as Shigella boydii 20. J Med Microbiol 56: 749-754 [Abstract] [Full Text]  
  • Talukder, K. A., Mondol, A. S., Islam, M. A., Islam, Z., Dutta, D. K., Khajanchi, B. K., Azmi, I. J., Hossain, M. A., Rahman, M., Cheasty, T., Cravioto, A., Nair, G. B., Sack, D. A. (2007). A novel serovar of Shigella dysenteriae from patients with diarrhoea in Bangladesh. J Med Microbiol 56: 654-658 [Abstract] [Full Text]  
  • Talukder, K. A., Islam, Z., Dutta, D. K., Islam, M. A., Khajanchi, B. K., Azmi, I. J., Iqbal, M. S., Hossain, M. A., Faruque, A. S. G., Nair, G. B., Sack, D. A. (2006). Antibiotic resistance and genetic diversity of Shigella sonnei isolated from patients with diarrhoea between 1999 and 2003 in Bangladesh.. J Med Microbiol 55: 1257-1263 [Abstract] [Full Text]  
  • Farshad, S., Sheikhi, R., Japoni, A., Basiri, E., Alborzi, A. (2006). Characterization of Shigella Strains in Iran by Plasmid Profile Analysis and PCR Amplification of ipa Genes.. J. Clin. Microbiol. 44: 2879-2883 [Abstract] [Full Text]  
  • Begum, Y. A., Talukder, K. A., Nair, G. B., Qadri, F., Sack, R. B., Svennerholm, A.-M. (2005). Enterotoxigenic Escherichia coli Isolated from Surface Water in Urban and Rural Areas of Bangladesh. J. Clin. Microbiol. 43: 3582-3583 [Full Text]  
  • Ansaruzzaman, M, Sultana, M., Talukder, K. A, Alam, K, Matsushita, S, Safa, A., Khajanchi, B. K, Dutta, D. K, Islam, Z., Albert, M J., Nair, G B., Sack, D. A (2005). Isolation and characterization of provisional serovar Shigella boydii E16553 from diarrhoeal patients in Bangladesh. J Med Microbiol 54: 477-480 [Abstract] [Full Text]  
  • Talukder, K. A, Khajanchi, B. K, Dutta, D. K, Islam, Z., Islam, M A., Iqbal, M S., Nair, G B., Sack, D. A (2005). An unusual cluster of dysentery due to Shigella dysenteriae type 4 in Dhaka, Bangladesh. J Med Microbiol 54: 511-513 [Full Text]  
  • Talukder, K. A., Khajanchi, B. K., Islam, M. A., Dutta, D. K., Islam, Z., Safa, A., Khan, G. Y., Alam, K., Hossain, M. A., Malla, S., Niyogi, S. K., Rahman, M., Watanabe, H., Balakrish Nair, G., Sack, D. A. (2004). Genetic relatedness of ciprofloxacin-resistant Shigella dysenteriae type 1 strains isolated in south Asia. J Antimicrob Chemother 54: 730-734 [Abstract] [Full Text]  
  • Talukder, K. A., Islam, M. A., Khajanchi, B. K., Dutta, D. K., Islam, Z., Safa, A., Alam, K., Hossain, A., Nair, G. B., Sack, D. A. (2003). Temporal Shifts in the Dominance of Serotypes of Shigella dysenteriae from 1999 to 2002 in Dhaka, Bangladesh. J. Clin. Microbiol. 41: 5053-5058 [Abstract] [Full Text]  
  • Talukder, K. A., Islam, Z., Aminul Islam, M., Dutta, D. K., Safa, A., Ansaruzzaman, M., Faruque, A. S. G., Shahed, S. N., Nair, G. B., Sack, D. A. (2003). Phenotypic and Genotypic Characterization of Provisional Serotype Shigella flexneri 1c and Clonal Relationships with 1a and 1b Strains Isolated in Bangladesh. J. Clin. Microbiol. 41: 110-117 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»