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Journal of Clinical Microbiology, July 2002, p. 2598-2600, Vol. 40, No. 7
0095-1137/02/$04.00+0     DOI: 10.1128/JCM.40.7.2598-2600.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.

Differentiation of Porcine Brachyspira Species by a Novel nox PCR-Based Restriction Fragment Length Polymorphism Analysis

Judith Rohde,* Anja Rothkamp, and Gerald F. Gerlach

Institut für Mikrobiologie und Tierseuchen, Tieraerztliche Hochschule Hannover, Hannover, Germany

Received 7 December 2001/ Returned for modification 10 March 2002/ Accepted 31 March 2002

A novel PCR-based restriction fragment length polymorphism analysis of the Brachyspira nox gene was developed. The restriction patterns for Brachyspira hyodysenteriae, B. pilosicoli, B. intermedia, B. murdochii, and B. innocens were highly distinct with two restriction endonucleases only. The assay proved to be user-friendly and robust.

* Corresponding author. Mailing address: Institut für Mikrobiologie und Tierseuchen, Tieraerztliche Hochschule Hannover, Bischofsholer Damm 15, D-30173 Hannover, Germany. Phone: 49 511 8567530. Fax: 49 511 856 7697. E-mail: Jurohde{at}gmx.de.

Journal of Clinical Microbiology, July 2002, p. 2598-2600, Vol. 40, No. 7
0095-1137/02/$04.00+0     DOI: 10.1128/JCM.40.7.2598-2600.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.





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