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Journal of Clinical Microbiology, July 2002, p. 2616-2617, Vol. 40, No. 7
0095-1137/02/$04.00+0     DOI: 10.1128/JCM.40.7.2616-2617.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.

Improvement of AMPLICOR Human Immunodeficiency Virus Type 1 Viral Load Test (Version 1.5) by Addition of a Coprecipitant during the RNA Isolation Step

Min Xu, Yung Chan, Steven H. Fischer, and Alan T. Remaley*

Department of Laboratory Medicine, National Institutes of Health, Bethesda, Maryland 20892-1508

Received 7 December 2001/ Returned for modification 14 March 2002/ Accepted 14 April 2002

The effect of the addition of a coprecipitant during the RNA isolation step on the analytical performance of the COBAS AMPLICOR human immunodeficiency virus type 1 (HIV-1) Monitor (version 1.5; Roche) viral load test was tested. Thirty-six specimens including patient samples, positive control samples, and negative control samples were processed in the presence and absence of the Pellet Paint coprecipitant. Specimens processed without the coprecipitant had lower RNA yields, as evidenced by a lower signal for the quantitation standard (QS). In addition, the results for all samples processed with the coprecipitant were acceptable on the basis of the optical density (OD) reading for the QS, whereas the result for one specimen processed without the coprecipitant was unacceptable on the basis of the OD reading for the QS, which required the assay to be repeated. Furthermore, the use of the coprecipitant improved the overall precision of the assay.

* Corresponding author. Mailing address: National Institutes of Health, Bldg. 10, Rm. 2C-433, 10 Center Dr., Bethesda, MD 20892-1508. Phone: (301) 402-9796. Fax: (301) 402-1885. E-mail: aremaley{at}nih.gov.

Journal of Clinical Microbiology, July 2002, p. 2616-2617, Vol. 40, No. 7
0095-1137/02/$04.00+0     DOI: 10.1128/JCM.40.7.2616-2617.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Xu, M., Chan, Y., Fischer, S. H., Remaley, A. T. (2004). Automated Procedure for Improving the RNA Isolation Step in Viral Load Testing for Human Immunodeficiency Virus. J. Clin. Microbiol. 42: 439-440 [Abstract] [Full Text]  


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