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Journal of Clinical Microbiology, August 2002, p. 2725-2728, Vol. 40, No. 8
0095-1137/02/$04.00+0     DOI: 10.1128/JCM.40.8.2725-2728.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.

PCR-Based Method for Detecting Viral Penetration of Medical Exam Gloves

John M. Broyles, Kevin P. O'Connell, and Denise M. Korniewicz*

Department of Adult Health Nursing, School of Nursing, University of Maryland, Baltimore, Maryland

Received 30 October 2001/ Returned for modification 24 February 2002/ Accepted 23 March 2002

The test approved by the U.S. Food and Drug Administration for assessment of the barrier quality of medical exam gloves includes visual inspection and a water leak test. Neither method tests directly the ability of gloves to prevent penetration by microorganisms. Methods that use microorganisms (viruses and bacteria) to test gloves have been developed but require classical culturing of the organism to detect it. We have developed a PCR assay for bacteriophage {phi}X174 that allows the rapid detection of penetration of gloves by this virus. The method is suitable for use with both latex and synthetic gloves. The presence of glove powder on either latex or synthetic gloves had no effect on the ability of the PCR assay to detect bacteriophage DNA. The assay is rapid, sensitive, and inexpensive; requires only small sample volumes; and can be automated.

* Corresponding author. Mailing address: School of Nursing, University of Maryland, Baltimore, 655 W. Lombard St., Baltimore, MD 21201. Phone: (410) 706-7250. Fax: (410) 706-2746. E-mail: dkorniew{at}son.umaryland.edu.

Journal of Clinical Microbiology, August 2002, p. 2725-2728, Vol. 40, No. 8
0095-1137/02/$04.00+0     DOI: 10.1128/JCM.40.8.2725-2728.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

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