This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Rosa e Silva, M. L.
Right arrow Articles by Gouvea, V.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Rosa e Silva, M. L.
Right arrow Articles by Gouvea, V.

 Previous Article  |  Next Article 

Journal of Clinical Microbiology, August 2002, p. 2837-2842, Vol. 40, No. 8
0095-1137/02/$04.00+0     DOI: 10.1128/JCM.40.8.2837-2842.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.

1998-1999 Rotavirus Seasons in Juiz de Fora, Minas Gerais, Brazil: Detection of an Unusual G3P[4] Epidemic Strain

Maria Luzia Rosa e Silva,1,2 Iná Pires de Carvalho,1 and Vera Gouvea1*

Department of Virology, Instituto de Microbiologia, Universidade Federal do Rio de Janeiro, Rio de Janeiro, RJ 21941-590,1 Department of Microbiology, Universidade Federal de Juiz de Fora, Juiz de Fora, MG 36036-330, Brazil2

Received 27 August 2001/ Returned for modification 29 December 2001/ Accepted 12 May 2002

An epidemiologic survey on the rotavirus strains causing gastroenteritis in young children was conducted in Juiz de Fora, Minas Gerais, in Southern Brazil during two consecutive seasons. Rotavirus was detected in 94 of the 1,056 fecal specimens collected from January 1998 to December 1999. Among the 13 discernible long electrophoretic profiles found, one was highly prevalent (73.4%) and represented the rotavirus strain responsible for the May-August winter epidemic outbreak of 1998, as clearly shown in a three-dimensional graph. This epidemic strain, designated JF98, was characterized as subgroup II and genotype G3P[4] by the original reverse transcription-PCR typing assays. Besides the unusual combination of G and P types, this G3 strain lacked reactivity with anti-G3-specific monoclonal antibodies and presented an uncommon pattern upon digestion of its cDNA-copied VP7 gene with the BstYI restriction enzyme. Strain JF98 affected primarily 6- to 24-month-old children and accounted for 85.5% of the severe rotavirus-associated dehydrating diarrhea cases that required hospitalization. As in our previous studies in neighboring Rio de Janeiro and São Paulo, a remarkably large proportion (44%) of mixed infections was detected, generating a complex set of circulating strains in the community, represented by the many distinct electropherotypes. Other common human types were detected as minor strains in single or in mixed infections, including the JF98 strain. Those were types G1, G4, G8, G9, P[8], and P[6], but not G2 or G5. One specimen contained a mixture of group A and C rotaviruses.

* Corresponding author. Mailing address: Molecular and Tropical Virology Laboratory, Department of Virology, IMPPG, UFRJ, Cidade Universitária, CCS, bloco I, Ilha do Fundão, Rio de Janeiro, 21941-590, Brazil. Phone: (55-21) 2260-9311. Fax: (55-21) 2560-8344. E-mail: vera.gouvea{at}micro.ufrj.br.

Journal of Clinical Microbiology, August 2002, p. 2837-2842, Vol. 40, No. 8
0095-1137/02/$04.00+0     DOI: 10.1128/JCM.40.8.2837-2842.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Hong, S.-K., Lee, S.-G., Lee, S.-A, Kang, J.-H., Lee, J.-H., Kim, J.-H., Kim, D.-S., Kim, H.-M., Jang, Y.-T., Ma, S.-H., Kim, S.-Y., Paik, S.-Y. (2007). Characterization of a G11,P[4] Strain of Human Rotavirus Isolated in South Korea. J. Clin. Microbiol. 45: 3759-3761 [Abstract] [Full Text]  
  • Castello, A. A., Arguelles, M. H., Rota, R. P., Olthoff, A., Jiang, B., Glass, R. I., Gentsch, J. R., Glikmann, G. (2006). Molecular Epidemiology of Group A Rotavirus Diarrhea among Children in Buenos Aires, Argentina, from 1999 to 2003 and Emergence of the Infrequent Genotype G12.. J. Clin. Microbiol. 44: 2046-2050 [Abstract] [Full Text]  
  • Santos, N., Volotao, E. M., Soares, C. C., Campos, G. S., Sardi, S. I., Hoshino, Y. (2005). Predominance of Rotavirus Genotype G9 during the 1999, 2000, and 2002 Seasons among Hospitalized Children in the City of Salvador, Bahia, Brazil: Implications for Future Vaccine Strategies. J. Clin. Microbiol. 43: 4064-4069 [Abstract] [Full Text]  
  • Santos, N., Soares, C. C., Volotao, E. M., Albuquerque, M. C. M., Hoshino, Y. (2003). Surveillance of Rotavirus Strains in Rio de Janeiro, Brazil, from 1997 to 1999. J. Clin. Microbiol. 41: 3399-3402 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»