This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Maddocks, S.
Right arrow Articles by Chen, S.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Maddocks, S.
Right arrow Articles by Chen, S.

 Previous Article  |  Next Article 

Journal of Clinical Microbiology, August 2002, p. 2999-3003, Vol. 40, No. 8
0095-1137/02/$04.00+0     DOI: 10.1128/JCM.40.8.2999-3003.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.

Comparison of CHROMagar Salmonella Medium and Xylose-Lysine-Desoxycholate and Salmonella-Shigella Agars for Isolation of Salmonella Strains from Stool Samples

Susan Maddocks,* Tom Olma, and Sharon Chen

Centre for Infectious Diseases and Microbiology Laboratory Services, Institute of Clinical Pathology and Medical Research, Westmead Hospital, Westmead, New South Wales, Australia

Received 2 January 2002/ Accepted 1 February 2002

The growth and appearance of 115 stock Salmonella isolates on a new formulation of CHROMagar Salmonella (CAS) medium were compared to those on xylose-lysine-desoxycholate agar (XLD), Salmonella-Shigella agar (SS), and Hektoen enteric agar (HEA) media. CAS medium was then compared prospectively to XLD and SS for the detection and presumptive identification of Salmonella strains in 500 consecutive clinical stool samples. All stock Salmonella isolates produced typical mauve colonies on CAS medium. Nine Salmonella strains were isolated from clinical specimens. The sensitivities for the detection of salmonellae after primary plating on CAS medium and the combination of XLD and SS after enrichment were 100%. The specificity for the detection of salmonellae after primary plating on CAS medium (83%) was significantly (P < 0.0001) higher than that after primary plating on the combination of SS and XLD media (55%) (a 28% difference in rates; 95% confidence interval, 23.0 to 34%). Twenty-nine non-Salmonella organisms produced mauve colonies on CAS medium, including 17 Candida spp. (59%) and 8 Pseudomonas spp. (28%). These were easily excluded as salmonellae by colony morphology, microscopic examination of a wet preparation, or oxidase testing. One biochemically inert Escherichia coli isolate required further identification to differentiate it from Salmonella spp. The use of plating on CAS medium demonstrated high levels of sensitivity and specificity and reduced the time to final identification of Salmonella spp., resulting in substantial cost savings. It can be recommended for use for the primary isolation of Salmonella spp. from stool specimens. Other media (e.g., XLD) are required to detect Shigella spp. concurrently.

* Corresponding author. Present address: Centre for Virus Research, Westmead Millenium Institute, Westmead Hospital, Hawkesbury Rd., Westmead 2145, Australia. Phone: 61-2-9845 9113. Fax: 61-2-9845 9103. E-mail: susan_maddocks{at}wmi.usyd.edu.au.

Journal of Clinical Microbiology, August 2002, p. 2999-3003, Vol. 40, No. 8
0095-1137/02/$04.00+0     DOI: 10.1128/JCM.40.8.2999-3003.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • van Dijk, S., Bruins, M. J., Ruijs, G. J. H. M. (2009). Evaluation and Implementation of a Chromogenic Agar Medium for Salmonella Detection in Stool in Routine Laboratory Diagnostics. J. Clin. Microbiol. 47: 456-458 [Abstract] [Full Text]  
  • Wilson, G. (2004). Rapid and Economical Method for Biochemical Screening of Stool Isolates for Salmonella and Shigella Species. J. Clin. Microbiol. 42: 4821-4823 [Abstract] [Full Text]  
  • Gray, S., Glancy, J., O'Hare, C., Doran, G., Cormican, M. (2003). Failure To Detect Salmonella enterica Serovar Dublin on Aes Laboratoire Salmonella Agar Plate. J. Clin. Microbiol. 41: 4003-4003 [Full Text]  
  • Perez, J. M., Cavalli, P., Roure, C., Renac, R., Gille, Y., Freydiere, A. M. (2003). Comparison of Four Chromogenic Media and Hektoen Agar for Detection and Presumptive Identification of Salmonella Strains in Human Stools. J. Clin. Microbiol. 41: 1130-1134 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»