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Journal of Clinical Microbiology, August 2002, p. 3079-3081, Vol. 40, No. 8
0095-1137/02/$04.00+0     DOI: 10.1128/JCM.40.8.3079-3081.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.

Effects of Repeated Subculturing and Prolonged Storage at Room Temperature of Enterohemorrhagic Escherichia coli O157:H7 on Pulsed-Field Gel Electrophoresis Profiles

Atsushi Iguchi,1 Ro Osawa,1* Junichi Kawano,2 Akira Shimizu,2 Jun Terajima,3 and Haruo Watanabe3

Department of Bioscience, Graduate School of Science and Technology,1 Department of Microbiology and Immunology, Faculty of Agriculture, Kobe University, Rokko-dai 1-1, Nada-ku, Kobe 657-8501,2 Department of Bacteriology, National Institute of Infectious Diseases, Toyama, Shinjuku-ku, Tokyo 162-8640, Japan3

Received 12 March 2002/ Returned for modification 6 May 2002/ Accepted 2 June 2002

Three clinical strains of enterohemorrhagic Escherichia coli O157:H7 which were subcultured repeatedly or stored at room temperature over a 25-week period showed appreciable variations in their pulsed-field gel electrophoresis fragment patterns. The variations could be explained by a couple of spontaneous genetic events at most and thus did not invalidate the genetic lineage of the strains.

* Corresponding author. Mailing address: Department of Bioscience, Graduate School of Science and Technology, Kobe University, Rokko-dai 1-1, Nada-ku, Kobe 657-8501, Japan. Phone and fax: 81-78-803-5804. E-mail: osawa{at}ans.kobe-u.ac.jp.

Journal of Clinical Microbiology, August 2002, p. 3079-3081, Vol. 40, No. 8
0095-1137/02/$04.00+0     DOI: 10.1128/JCM.40.8.3079-3081.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.



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