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Journal of Clinical Microbiology, August 2002, p. 3093-3097, Vol. 40, No. 8
0095-1137/02/$04.00+0     DOI: 10.1128/JCM.40.8.3093-3097.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.

Genotyping of Methicillin-Resistant Staphylococcus aureus by Assaying for the Presence of Variable Elements Associated with mecA

Flavia Huygens,1 Graeme R. Nimmo,2 Jacqueline Schooneveldt,2 Wendy J. Munckhof,3 and Philip M. Giffard1*

Cooperative Research Centre for Diagnostics, Queensland University of Technology, Brisbane, Queensland 4001,1 Microbiology Department, Queensland Health Pathology Service,2 Infection Management Services Southern Queensland, Princess Alexandra Hospital, Woolloongabba, Queensland 4102, Australia3

Received 9 October 2001/ Returned for modification 5 December 2001/ Accepted 13 May 2002

The region surrounding mecA in methicillin-resistant Staphylococcus aureus (MRSA) is highly variable. We describe an approach for the rapid genotyping of MRSA by assaying for the presence or absence of variable or mobile elements previously shown to be associated with the mecA region.

* Corresponding author. Mailing address: Cooperative Research Centre for Diagnostics, Queensland University of Technology, GPO Box 2434, Brisbane, Queensland 4001, Australia. Phone: 61 7 3864 2015. Fax: 61 7 3864 1534. E-mail: p.giffard{at}qut.edu.au.

Journal of Clinical Microbiology, August 2002, p. 3093-3097, Vol. 40, No. 8
0095-1137/02/$04.00+0     DOI: 10.1128/JCM.40.8.3093-3097.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Stephens, A. J., Huygens, F., Inman-Bamber, J., Price, E. P., Nimmo, G. R., Schooneveldt, J., Munckhof, W., Giffard, P. M. (2006). Methicillin-resistant Staphylococcus aureus genotyping using a small set of polymorphisms. J Med Microbiol 55: 43-51 [Abstract] [Full Text]  
  • van der Zee, A., Heck, M., Sterks, M., Harpal, A., Spalburg, E., Kazobagora, L., Wannet, W. (2005). Recognition of SCCmec Types According to Typing Pattern Determined by Multienzyme Multiplex PCR-Amplified Fragment Length Polymorphism Analysis of Methicillin-Resistant Staphylococcus aureus. J. Clin. Microbiol. 43: 6042-6047 [Abstract] [Full Text]  
  • Huygens, F., Stephens, A. J., Nimmo, G. R., Giffard, P. M. (2004). mecA Locus Diversity in Methicillin-Resistant Staphylococcus aureus Isolates in Brisbane, Australia, and the Development of a Novel Diagnostic Procedure for the Western Samoan Phage Pattern Clone. J. Clin. Microbiol. 42: 1947-1955 [Abstract] [Full Text]  


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