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Journal of Clinical Microbiology, September 2002, p. 3341-3345, Vol. 40, No. 9
0095-1137/02/$04.00+0     DOI: 10.1128/JCM.40.9.3341-3345.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.

High Prevalence of Human Papillomavirus (HPV) Infections and High Frequency of Multiple HPV Genotypes in Human Immunodeficiency Virus-Infected Women in Brazil

José E. Levi,1 Bernhard Kleter,2 Wim G. V. Quint,2 Maria C. S. Fink,1 Cynthia L. M. Canto,1 Regina Matsubara,3 Iara Linhares,4 Aluísio Segurado,1,4 Bart Vanderborght,5,6 José Eluf Neto,7 and Leen-Jan van Doorn2*

Laboratório de Virologia do Instituto de Medicina Tropical,1 Instituto de Infectologia Emílio Ribas,3 Núcleo de Extensão ao Atendimento ao Paciente HIV+ e c/AIDS do Departamento de Doenças Infecciosas e Parasitárias,4 Departamento de Medicina Preventiva, Faculdade de Medicina da Universidade de São Paulo, São Paulo,7 Hospital Universitario Clementina Frago Filho, Universidade Federal de Rio de Janeiro, Rio de Janeiro, Brazil,6 Delft Diagnostic Laboratory, Delft, The Netherlands,2 Innogenetics, Ghent, Belgium5

Received 1 October 2001/ Returned for modification 25 March 2002/ Accepted 14 June 2002

A group of 208 human immunodeficiency virus (HIV)-infected women in Brazil were studied for the presence of human papillomavirus with the general SPF10 PCR primer set. Virtually all (98%) women were found positive for human papillomavirus (HPV) DNA. Genotyping by the reverse hybridization line probe assay (HPV-LiPA) revealed a high prevalence of multiple genotypes (78.9% of the cases), with an average of 3.1 genotypes per patient (range, 1 to 10 genotypes). HPV 6 was the most prevalent genotype and was observed in 80 (39.2%) patients, followed by types 51 (31.9%), 11 (26.0%), 18 (24.0%), and 16 (22.5%). Of the genotypes detected, 40.9% were low-risk genotypes. Twenty-two (10.5%) patients showed normal (Pap I) cytology, 149 (71.6%) patients had inflammation (Pap II), and 28 patients (13.4%) had a Pap III score. The prevalence of high-risk genotypes increased with the cytological classification. There were no significant associations between the number of HPV genotypes detected and the cytological classification, HIV viral load, and CD4 count in these patients. In conclusion, the highly sensitive SPF10 LiPA system shows that a very high proportion of HIV-infected women in Brazil are infected with HPV and often carry multiple HPV genotypes.

* Corresponding author. Mailing address: Delft Diagnostic Laboratory, 2625 AD Delft, The Netherlands. Phone: 31 15 2604581. Fax: 31 15 2604550. E-mail: L.J.van.Doorn{at}ddl.nl.

Journal of Clinical Microbiology, September 2002, p. 3341-3345, Vol. 40, No. 9
0095-1137/02/$04.00+0     DOI: 10.1128/JCM.40.9.3341-3345.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.



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