This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Smole, S. C.
Right arrow Articles by Arbeit, R. D.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Smole, S. C.
Right arrow Articles by Arbeit, R. D.

 Previous Article  |  Next Article 

Journal of Clinical Microbiology, September 2002, p. 3374-3380, Vol. 40, No. 9
0095-1137/02/$04.00+0     DOI: 10.1128/JCM.40.9.3374-3380.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.

Clinical and Epidemiological Correlates of Genotypes within the Mycobacterium avium Complex Defined by Restriction and Sequence Analysis of hsp65

Sandra C. Smole,1,2,3 Fionnuala McAleese,3 Jutamas Ngampasutadol,4 C. Fordham von Reyn,5 and Robert D. Arbeit1,3,4*

Departments of Medicine,1 Microbiology, Boston University School of Medicine,4 Department of Epidemiology, Boston University School of Public Health,2 Infectious Diseases Section, Medical Service, VA Boston Healthcare System, Boston, Massachusetts 02130,3 Department of Medicine, Dartmouth-Hitchcock Medical Center and Dartmouth Medical School, Hanover, New Hampshire 037565

Received 31 January 2002/ Returned for modification 21 April 2002/ Accepted 9 June 2002

Species identification of isolates of the Mycobacterium avium complex (MAC) remains a difficult task. Although M. avium and Mycobacterium intracellulare can be identified with expensive, commercially available probes, many MAC isolates remain unresolved, including those representing Mycobacterium lentiflavum as well as other potentially undefined species. PCR restriction analysis (PRA) of the hsp65 gene has been proposed as a rapid and inexpensive approach. We applied PRA to 278 MAC isolates, including 126 from blood of human immunodeficiency virus (HIV)-infected patients, 59 from sputum of HIV-negative patients with chronic obstructive pulmonary disease, 88 from environmental sources, and 5 pulmonary isolates from a different study. A total of 15 different PRA patterns were observed. For 27 representative isolates, a 441-bp fragment of the hsp65 gene was sequenced; based on 54 polymorphic sites, 18 different alleles were defined, including 12 alleles not previously reported. Species and phylogenetic relationships were more accurately defined by sequencing than by PRA or commercial probe. The distribution of PRA types and, by implication, phylogenetic lineages among blood isolates was significantly different from that for pulmonary and environmental isolates, suggesting that particular lineages have appreciably greater virulence and invasive potential.

* Corresponding author. Present address: Cubist Pharmaceuticals, Inc., 65 Hayden Dr., Lexington, MA 02420. Phone: (781) 860-8304. Fax: (617) 278-4540. E-mail: robert.arbeit{at}cubist.com.

Journal of Clinical Microbiology, September 2002, p. 3374-3380, Vol. 40, No. 9
0095-1137/02/$04.00+0     DOI: 10.1128/JCM.40.9.3374-3380.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • van Ingen, J, Bendien, S A, de Lange, W C M, Hoefsloot, W, Dekhuijzen, P N R, Boeree, M J, van Soolingen, D (2009). Clinical relevance of non-tuberculous mycobacteria isolated in the Nijmegen-Arnhem region, The Netherlands. Thorax 64: 502-506 [Abstract] [Full Text]  
  • Mackenzie, N., Alexander, D. C., Turenne, C. Y., Behr, M. A., De Buck, J. M. (2009). Genomic Comparison of PE and PPE Genes in the Mycobacterium avium Complex. J. Clin. Microbiol. 47: 1002-1011 [Abstract] [Full Text]  
  • Pfaller, S. L., Aronson, T. W., Holtzman, A. E., Covert, T. C. (2007). Amplified fragment length polymorphism analysis of Mycobacterium avium complex isolates recovered from southern California. J Med Microbiol 56: 1152-1160 [Abstract] [Full Text]  
  • Turenne, C. Y., Wallace, R. Jr., Behr, M. A. (2007). Mycobacterium avium in the Postgenomic Era. Clin. Microbiol. Rev. 20: 205-229 [Abstract] [Full Text]  
  • Griffith, D. E., Brown-Elliott, B. A., Langsjoen, B., Zhang, Y., Pan, X., Girard, W., Nelson, K., Caccitolo, J., Alvarez, J., Shepherd, S., Wilson, R., Graviss, E. A., Wallace, R. J. Jr. (2006). Clinical and Molecular Analysis of Macrolide Resistance in Mycobacterium avium Complex Lung Disease. Am. J. Respir. Crit. Care Med. 174: 928-934 [Abstract] [Full Text]  
  • Prammananan, T., Phunpruch, S., Tingtoy, N., Srimuang, S., Chaiprasert, A. (2006). Distribution of hsp65 PCR-Restriction Enzyme Analysis Patterns among Mycobacterium avium Complex Isolates in Thailand. J. Clin. Microbiol. 44: 3819-3821 [Abstract] [Full Text]  
  • Murcia, M. I., Tortoli, E., Menendez, M. C., Palenque, E., Garcia, M. J. (2006). Mycobacterium colombiense sp. nov., a novel member of the Mycobacterium avium complex and description of MAC-X as a new ITS genetic variant.. Int. J. Syst. Evol. Microbiol. 56: 2049-2054 [Abstract] [Full Text]  
  • Turenne, C. Y., Semret, M., Cousins, D. V., Collins, D. M., Behr, M. A. (2006). Sequencing of hsp65 Distinguishes among Subsets of the Mycobacterium avium Complex. J. Clin. Microbiol. 44: 433-440 [Abstract] [Full Text]  
  • Wu, C.-w., Glasner, J., Collins, M., Naser, S., Talaat, A. M. (2006). Whole-Genome Plasticity among Mycobacterium avium Subspecies: Insights from Comparative Genomic Hybridizations. J. Bacteriol. 188: 711-723 [Abstract] [Full Text]  
  • Kim, H., Kim, S.-H., Shim, T.-S., Kim, M.-n., Bai, G.-H., Park, Y.-G., Lee, S.-H., Chae, G.-T., Cha, C.-Y., Kook, Y.-H., Kim, B.-J. (2005). Differentiation of Mycobacterium species by analysis of the heat-shock protein 65 gene (hsp65). Int. J. Syst. Evol. Microbiol. 55: 1649-1656 [Abstract] [Full Text]  
  • Huang, T.-S., Lee, S. S.-J., Chen, Y.-S., Tu, H.-Z., Huang, W.-K., Liu, Y.-C. (2005). Discordant molecular characterization results in a Mycobacterium avium complex strain isolated from an AIDS patient. J Med Microbiol 54: 681-683 [Abstract] [Full Text]  
  • Lebrun, L., Weill, F.-X., Lafendi, L., Houriez, F., Casanova, F., Gutierrez, M. C., Ingrand, D., Lagrange, P., Vincent, V., Herrmann, J. L. (2005). Use of the INNO-LiPA-MYCOBACTERIA Assay (Version 2) for Identification of Mycobacterium avium-Mycobacterium intracellulare-Mycobacterium scrofulaceum Complex Isolates. J. Clin. Microbiol. 43: 2567-2574 [Abstract] [Full Text]  
  • Naughton, J. F., Mealey, K. L., Wardrop, K. J., Oaks, J. L., Bradway, D. S. (2005). Systemic Mycobacterium avium Infection in a Dog Diagnosed by Polymerase Chain Reaction Analysis of Buffy Coat. Journal of the American Animal Hospital Association 41: 128-132 [Abstract] [Full Text]  
  • McNabb, A., Eisler, D., Adie, K., Amos, M., Rodrigues, M., Stephens, G., Black, W. A., Isaac-Renton, J. (2004). Assessment of Partial Sequencing of the 65-Kilodalton Heat Shock Protein Gene (hsp65) for Routine Identification of Mycobacterium Species Isolated from Clinical Sources. J. Clin. Microbiol. 42: 3000-3011 [Abstract] [Full Text]  
  • Turenne, C. Y., Thibert, L., Williams, K., Burdz, T. V., Cook, V. J., Wolfe, J. N., Cockcroft, D. W., Kabani, A. (2004). Mycobacterium saskatchewanense sp. nov., a novel slowly growing scotochromogenic species from human clinical isolates related to Mycobacterium interjectum and Accuprobe-positive for Mycobacterium avium complex. Int. J. Syst. Evol. Microbiol. 54: 659-667 [Abstract] [Full Text]  
  • Oliveira, R. S., Sircili, M. P., Oliveira, E. M. D., Balian, S. C., Ferreira-Neto, J. S., Leao, S. C. (2003). Identification of Mycobacterium avium Genotypes with Distinctive Traits by Combination of IS1245-Based Restriction Fragment Length Polymorphism and Restriction Analysis of hsp65. J. Clin. Microbiol. 41: 44-49 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»