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Journal of Clinical Microbiology, September 2002, p. 3512-3514, Vol. 40, No. 9
0095-1137/02/$04.00+0     DOI: 10.1128/JCM.40.9.3512-3514.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.

Simple and Reliable Method for Detection and Genotyping of Hepatitis C Virus RNA in Dried Blood Spots Stored at Room Temperature

Mariacarmela Solmone,1 Enrico Girardi,1 Francesco Costa,2 Leopoldo Pucillo,1 Giuseppe Ippolito,1 and Maria R. Capobianchi1*

National Institute for Infectious Diseases L. Spallanzani, IRCCS,1 Central Laboratory Italian Red Cross, Rome, Italy2

Received 13 December 2001/ Returned for modification 14 April 2002/ Accepted 18 May 2002

We describe a simple, sensitive, and reproducible method for using whole blood collected onto filter paper (dried blood spots) for detection and genotyping of hepatitis C virus RNA that can be useful in large field studies, particularly in settings where collection, preparation, storage, and shipment of samples at controlled temperature can be difficult.

* Corresponding author. Mailing address: Laboratory of Virology, National Institute of Infectious Diseases L. Spallanzani, Via Portuense 292, 00149 Rome, Italy. Phone: 39 0655170434. Fax: 39 065582346. E-mail: mrcapobianchi{at}libero.it.

Journal of Clinical Microbiology, September 2002, p. 3512-3514, Vol. 40, No. 9
0095-1137/02/$04.00+0     DOI: 10.1128/JCM.40.9.3512-3514.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.



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