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Journal of Clinical Microbiology, September 2002, p. 3540-3541, Vol. 40, No. 9
0095-1137/02/$04.00+0     DOI: 10.1128/JCM.40.9.3540-3541.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.

LETTER TO THE EDITOR

Enteroaggregative Escherichia coli Strains among Classical Enteropathogenic Escherichia coli O Serogroups

Top Letter References

 
Enteroaggregative Escherichia coli (EAEC) is an emerging cause of diarrhea in both developing and developed countries (6) that is defined by the characteristic pattern of aggregative adherence (AA) to cultured epithelial cells (7). Studies from various laboratories have demonstrated that some enteropathogenic E. coli (EPEC) serogroups are comprised of serotypes mostly consisting of EAEC strains (2, 5, 9, 10). These serotypes present interesting characteristics: i.e., they have been isolated from cases of acute infantile diarrhea before AA was recognized as a distinct adherence pattern and in most cases express AA only in the 6-h adherence assay (5, 9, 10).

We examined our laboratory collection of E. coli strains that present the AA phenotype to HEp-2 cells and belong to the EPEC O serogroups (L. R. Trabulsi, unpublished data) for the presence of EAEC-associated virulence markers (3). Thirty-four AA-producing strains of the following serotypes were selected: O86:H2, O111:H4, O111:H10, O111:H12, O111:H21, O125:H6, O125:H16, O125:H21, O126:H27, O128:H12, and O128:H35. The strains were first analyzed for the presence of intimin (eae) and bundle-forming pilus structural subunit (bfpA) genes of EPEC (6). PCR for eae was performed as described earlier (1). The primers employed for bfpA detection (forward, 5'-GGTCTGTCTTTGATTGAATC-3'; and reverse, 5'-TTTACATGCAGTTGCCGCTT-3') were based on its published sequence (GenBank accession no. NC_002142) in order to amplify a 485-bp fragment. The presence of plasmid (aggA, aafA, aggR, astA, pet, aspU, and shf) and chromosomal (irp2 and pic) EAEC-associated virulence markers (3) and the EAEC probe sequence (8) were also investigated by PCR, as previously described (3, 4, 8).

All strains evaluated were negative for the presence of EPEC virulence genes, except the two O125:H6 strains, which harbored the eae gene. Interestingly, these latter strains lacked all EAEC markers evaluated (Table 1) and presented the AA pattern to HEp-2 cells in the 6-h adhesion assay. All other serotypes studied presented at least one EAEC marker (Table 1). The most prevalent markers found were irp2, astA, shf, and pic, which have also been described as prevalent among EAEC strains (3). The aggregative adherence fimbrial I pilin (aggA) gene (6) was the only EAEC marker not detected. This is in accordance with the low prevalence of aggA found in another study (4).

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TABLE 1. Detection of EAEC- and EPEC-associated virulence markers among E. coli strains belonging to EPEC serogroups and presenting the AA phenotype

EAEC is very heterogeneous regarding the presence of putative virulence markers, and although some of these markers are frequent in the category, a sensitive gene probe for EAEC identification is not yet available (6). Accordingly, adherence to epithelial cells remains the "gold standard" diagnostic test for the category (7). Our results demonstrated that, with the exception of O125:H6, which has been classified as atypical EPEC (10), the serotypes studied here should be considered to be EAEC, since they presented the AA pattern and the majority of the EAEC-associated virulence genes. Studies in our laboratories are in progress in order to characterize the adhesin mediating the AA phenotype of the O125:H6 serotype and to ascertain whether these strains are able to produce the characteristic attaching-and-effacing lesion of EPEC (6). Our findings have clinical and epidemiological relevance, since EPEC identification by O serogrouping is misleading, as EPEC serogroups include different pathogens. Therefore, O sero-grouping should be complemented by flagellar (H) typing or, alternatively, by testing adhesion to epithelial cells.

Top Letter References

 

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1. Batchelor, M., S. Knutton, A. Caprioli, V. Huter, M. Zanial, G. Dougan, and G. Frankel. 1999. Development of a universal intimin antiserum and PCR primers. J. Clin. Microbiol. 37:3822-3827.[Abstract/Free Full Text]
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2. Campos, L. C., T. S. Whittam, T. A. T. Gomes, J. R. C. Andrade, and L. R. Trabulsi. 1994. Escherichia coli serogroup O111 includes several clones of diarrheagenic strains with different virulence properties. Infect. Immun. 62:3282-3288.[Abstract/Free Full Text]
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3. Czeczulin, J. R., T. S. Whittam, I. R. Henderson, F. Navarro-Garcia, and J. P. Nataro. 1999. Phylogenetic analysis of enteroaggregative and diffusely adherent Escherichia coli. Infect. Immun. 67:2692-2699.[Abstract/Free Full Text]
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4. Gioppo, N. M. R., W. P. Elias, Jr., M. C. Vidotto, R. E. Linhares, H. O. Saridakis, T. A. T. Gomes, L. R. Trabulsi, and J. S. Pelayo. 2000. Prevalence of HEp-2 cell-adherent Escherichia coli and characterization of enteroaggregative E. coli and chain-like adherent E. coli isolated from children with and without diarrhoea, in Londrina, Brazil. FEMS Microbiol. Lett. 190:293-298.[CrossRef][Medline]
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5. Monteiro-Neto, V., L. C. Campos, A. J. P. Ferreira, T. A. T. Gomes, and L. R. Trabulsi. 1997. Virulence properties of Escherichia coli O111:H12 strains. FEMS Microbiol. Lett. 146:123-128.[CrossRef][Medline]
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6. Nataro, J. P., and J. B. Kaper. 1998. Diarrheagenic Escherichia coli. Clin. Microbiol. Rev. 11:142-201.[Abstract/Free Full Text]
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7. Nataro, J. P., J. B. Kaper, R. Robins-Browne, V. Prado, P. Vial, and M. M. Levine. 1987. Patterns of adherence of diarrheagenic Escherichia coli to HEp-2 cells. Pediatr. Infect. Dis. J. 6:829-831.[Medline]
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8. Schmidt, H., C. Knop, S. Franke, S. Aleksic, J. Heesemann, and H. Karch. 1995. Development of PCR for screening of enteroaggregative Escherichia coli. J. Clin. Microbiol. 33:701-705.[Abstract]
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9. Scotland, S. M., H. R. Smith, T. Cheasty, B. Said, G. A. Willshaw, N. Stokes, and B. Rowe. 1996. Use of gene probes and adhesion tests to characterise Escherichia coli belonging to enteropathogenic serogroups isolated in the United Kingdom. J. Med. Microbiol. 44:438-443.[Abstract/Free Full Text]
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10. Valle, G. R. F., T. A. T. Gomes, K. Irino, and L. R. Trabulsi. 1997. The traditional enteropathogenic Escherichia coli (EPEC) serogroup O125 comprises serotypes which are mainly associated with the category of enteroaggregative E. coli. FEMS Microbiol. Lett. 152:95-100.[CrossRef][Medline]

						Waldir P. Elias* Samar F. Barros Cristiano G. Moreira Luiz R. Trabulsi Laboratório Especial de Microbiologia Instituto Butantan 05503-900, São Paulo, SP, Brazil Tânia A. T. Gomes Departamento de Microbiologia, Imunologia e Parasitologia Universidade Federal de São Paulo 04023-062, São Paulo, SP, Brazil
						* Phone: (5511) 3726-7222, ext. 2075 Fax: (5511) 3726-1505 E-mail: wpelias{at}usp.br.

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Journal of Clinical Microbiology, September 2002, p. 3540-3541, Vol. 40, No. 9
0095-1137/02/$04.00+0     DOI: 10.1128/JCM.40.9.3540-3541.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.

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