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Journal of Clinical Microbiology, January 2003, p. 439-441, Vol. 41, No. 1
0095-1137/03/$08.00+0     DOI: 10.1128/JCM.41.1.439-441.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.

Use of Real-Time PCR To Detect and Quantify Mycoplasma haemofelis and "Candidatus Mycoplasma haemominutum" DNA

Séverine Tasker,* Chris R. Helps, Michael J. Day, Tim J. Gruffydd-Jones, and Dave A. Harbour

Department of Clinical Veterinary Science, University of Bristol, Langford House, Langford, Bristol, BS40 5DU, United Kingdom

Received 15 May 2002/ Returned for modification 22 July 2002/ Accepted 1 October 2002

A real-time PCR assay using Taqman probes was developed to detect and quantify Mycoplasma haemofelis and "Candidatus Mycoplasma haemominutum" in feline blood samples. The assay was rapid and sensitive and was successfully used to monitor the in vivo kinetics of cats experimentally infected with each species.

* Corresponding author. Mailing address: Department of Clinical Veterinary Science, University of Bristol, Langford House, Langford, Bristol, BS40 5DU, United Kingdom. Phone: 44 117 928 9280. Fax: 44 117 928 9505. E-mail: s.tasker{at}bristol.ac.uk.

Journal of Clinical Microbiology, January 2003, p. 439-441, Vol. 41, No. 1
0095-1137/03/$08.00+0     DOI: 10.1128/JCM.41.1.439-441.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.



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